FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences764838
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC25260.3302660171173504No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAAAGTCGCCG16180.21154806638791482No Hit
GTAATACACGGCAGTGTCGTCAGCCTTTAGGCTGCTGATCTGCAGATATG16100.21050209325373476No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT12910.16879391452830533No Hit
TCCATAGCCTGGGTCCCGCTGATGGTCAGAGTGGCTGTGTTCCCAGAGTT12910.16879391452830533No Hit
GCTCCACACCATGGACTGGACCTGGAGGATCCTCTTCTTGGTGGCAGCAG12600.16474076863335765No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC12600.16474076863335765No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA12510.1635640488574051No Hit
GTGTATTACTGTGCGAGACACAGGAACAACTACGGTGACTATTATTACTA12390.16199508915613503No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA11590.15153535781433453No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT11580.15140461117256204No Hit
CCTATGAGCTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACA11570.15127386453078953No Hit
CCCCTGTGCTGGTCATCTATCAAGATTTCAAGCGGCCCTCAGGGATCCCT11480.15009714475483696No Hit
CTATTATTACTACGGCATGGACGTCTGGGGCCAAGGGACCACGGTCACCG11320.14800519848647686No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11190.1463054921434343No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT10510.1374147205029039No Hit
GTAATAATAGTCACCGTAGTTGTTCCTGTGTCTCGCACAGTAATACACGG10490.13715322721935888No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC10330.13506128095099876No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9840.12865469550414596No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA9760.12760872236996593No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT9710.1269549891611034No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC9540.12473229625097079No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA9290.12146363020665814No Hit
GGCTATGGATGAGGCTGACTATTTCTGTCAGGCGTGGGGCGGCGACTTTT9210.12041765707247809No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAAAGTCGCCGC9210.12041765707247809No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA9190.12015616378893308No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT9100.11897944401298054No Hit
GTGCTGGTCATCTATCAAGATTTCAAGCGGCCCTCAGGGATCCCTGAGCG8940.11688749774462044No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC8910.11649525781930292No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC8900.11636451117753041No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA8780.11479555147626035No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG8770.11466480483448784No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA8620.11270360520790025No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC8400.10982717908890509No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA8300.10851971267118003No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG8170.10682000632813746No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA8140.10642776640281994No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA8110.10603552647750242No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG8030.10498955334332237No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG8000.10459731341800485No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT7980.10433582013445984No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT7710.10080566080660218No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTC450.00990870447.995957
ATCAACG31200.041.3042033
TCAACGC31400.040.811844
GTATCAA32600.040.2798231
TATCAAC32700.039.437882
CAACGCA32650.039.2493675
AACGCAG32600.039.0887226
ACGCAGA34300.036.9414867
GTGTAGA4500.035.2731251
CGCAGAG36000.035.1970258
GCAGAGT37900.033.2425779
TCTACAC5350.032.2963373
TATTGAG8350.029.3148925
GTGCTAG4050.028.5035381
CTAGGTC14050.027.157853
GAATACG3255.638867E-1126.582379
CCTAGGT14350.026.1071622
GTATATA4500.025.6531811
ATTGAGT7050.025.5297586
TGTAGAG6300.025.1588272
Produced by FastQC (version 0.11.5)