FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2101668
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA59690.28401250816018514No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC54350.2586041182527402No Hit
GGTTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACT53910.25651054305437393No Hit
GATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCAT52070.24775559222484236No Hit
GTAATAATCAGCCTCATCCTCACCCTGGAGCCCAGTGATGGCCAGGGAGG50490.2402377540125272No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT50390.23976194146744395No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC49120.2337191221448868No Hit
CTCCTGCACTGGGAGCAGCTCCAACTTCGGGGCAGGTTATGATGTACACT46910.22320366489854723No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT46170.2196826520649313No Hit
GATTATTACTGCCAGTCCTATGACACCAGCCTGCGTGCTTGGGTGTTCGG45760.21773182063009No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA44580.21211723259810777No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA41490.19741462495503573No Hit
TCCAGGAACAGCCCCCAAACTCCTCATCTCTGGTAACACCAATCGGCCCT39430.187612886526321No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA38540.1833781548750802No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA37720.17947649200539761No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT36740.17481352906358189No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT36290.1726723726107073No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA36240.1724344663381657No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG35330.16810457217790822No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA34500.16415532805371733No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT34080.16215691536436772No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG33740.1605391527110847No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA33070.15735120865902702No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG32670.15544795847869408No Hit
CCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAAGTGCCAGACTTGGAGCC32600.1551148896971358No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA31790.15126080808196157No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG30220.1437905511241547No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG29700.14131632588972187No Hit
GTTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTC29180.13884210065528904No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC29120.1385566131282391No Hit
GGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCATCTCTGGTAAC29070.13831870685569747No Hit
TCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC26810.1275653433368163No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT26600.12656613699214148No Hit
ACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGCACGCAGGC26590.12651855573763315No Hit
CCCAAGCACGCAGGCTGGTGTCATAGGACTGGCAGTAATAATCAGCCTCA26510.12613790570156655No Hit
TCCTCACCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAAGTGCCAGACTT26500.12609032444705826No Hit
GGCTGGTGTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCACCCTGG26140.12437739928475859No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG25450.12109429272368424No Hit
TCATAGGACTGGCAGTAATAATCAGCCTCATCCTCACCCTGGAGCCCAGT24790.11795392992613486No Hit
ACACCAGCCTGCGTGCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTC23720.11286273569374422No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC23430.11148287931300283No Hit
TCTCTGGTAACACCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGC23360.11114981053144456No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGC23290.11081674174988627No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA23100.10991269791422813No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGT22980.10934172286012823No Hit
TCACTGGGCTCCAGGGTGAGGATGAGGCTGATTATTACTGCCAGTCCTAT22940.10915139784209495No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC22830.10862800404250338No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT22310.10615377880807055No Hit
GTGTACATCATAACCTGCCCCGAAGTTGGAGCTGCTCCCAGTGCAGGAGA22270.10596345379003726No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG22200.10563038500847899No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGG22160.10544005999044569No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC22160.10544005999044569No Hit
GTCCTATGACACCAGCCTGCGTGCTTGGGTGTTCGGCGGAGGGACCAAGC21920.1042981098822459No Hit
GCCTCATCCTCACCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAAGTGCC21810.10377471608265436No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACTTCAG21540.10249002221092961No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGC21390.10177630339330475No Hit
ATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTC21330.1014908158662548No Hit
CTGGTAACACCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCC21290.10130049084822151No Hit
GACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGCACGCAGG21210.10091984081215491No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA39550.059.8613131
ATCAACG40250.058.307213
TCAACGC41100.057.1013374
TATCAAC44050.053.3293152
CAACGCA46400.050.7341355
AACGCAG47700.049.3514486
CGCAGAG48700.048.338078
ACGCAGA52050.045.226977
GCAGAGT59000.040.0214089
GGTATCA18850.038.2919541
GTACATG50850.036.6224751
ACATGGG52650.035.140283
TACATGG52300.035.1344222
TATTGAG35650.032.309555
CATGGGG35800.031.1687364
TTATTGA40400.027.7980124
TACCGTC2156.520022E-726.7868737
ATTGAGT25550.025.3584146
CAGAGTA56500.024.9734279
TAGCGCG1751.3180806E-424.6821924
Produced by FastQC (version 0.11.5)