FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280735

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280735
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1148475
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG38840.3381875965954853No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT34010.2961318269879623No Hit
CCTATGAGCTGACTCAGCCACCCTCAGTGTCAGTGGCCCCAGGAGAGACG33940.2955223230806069No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA31090.2707068068525653No Hit
CTTCTAATATCGACCGGCCCTCAGGGATCCCTGAGCGCTTCTCTGGCTCC28580.2488517381745358No Hit
TACTGATACTGATCATCCGGGGGTCTTCGGATCTGGGACTAAGGTCACCG28420.24745858638629487No Hit
GTATCAGTATTCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTC27810.24214719519362635No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC26840.2337012124774157No Hit
GCCCTCAGGGATCCCTGAGCGCTTCTCTGGCTCCAATTCTGGGAACACGG25510.22212063823766298No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA25020.21785411088617515No Hit
ACCCAGGACGGTGACCTTAGTCCCAGATCCGAAGACCCCCGGATGATCAG23780.20705718452730795No Hit
GTCCTGGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC23550.20505452883171163No Hit
CCCCTGTGCTGGTCATCTCTTCTAATATCGACCGGCCCTCAGGGATCCCT23130.20139750538757917No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT22940.19974313763904306No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC22160.19295152267136856No Hit
GCCTTGGGCTGACCCAGGACGGTGACCTTAGTCCCAGATCCGAAGACCCC21280.18528918783604345No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC21200.18459261194192297No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA20810.18119680445808573No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC19280.16787479048303186No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG18960.16508848690654998No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT18490.16099610352859225No Hit
GTACATGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCA18490.16099610352859225No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGAGCTGACTCAGCCAC16940.14749994558000826No Hit
ATATTAGAAGAGATGACCAGCACAGGGGCCTGGCCTGGCTTCTGCTGGTA16750.14584557783147217No Hit
GCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAATGCACACTTTTTCTT16430.14305927425499032No Hit
CCTGAGGGCCGGTCGATATTAGAAGAGATGACCAGCACAGGGGCCTGGCC16410.14288513028146022No Hit
GGCTCTGTGACCTCCTATGAGCTGACTCAGCCACCCTCAGTGTCAGTGGC16390.14271098630793008No Hit
TCCTGGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC16380.142623914321165No Hit
GCACAGGCTCTGTGACCTCCTATGAGCTGACTCAGCCACCCTCAGTGTCA16120.14036004266527352No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA15450.13452621955201463No Hit
GTGCTGGTCATCTCTTCTAATATCGACCGGCCCTCAGGGATCCCTGAGCG15390.1340037876314243No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT14800.12886654041228585No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG14550.1266897407431594No Hit
CCCCAGGAGAGACGGCCAGGATTGCCTGTGGGGGAAACAACATTGGAAGA14050.1223361414049065No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT13960.12155249352402099No Hit
GACCCAGGACGGTGACCTTAGTCCCAGATCCGAAGACCCCCGGATGATCA13930.1212912775637258No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACAC13310.11589281438429222No Hit
GGGTGGCCGTGTTCCCAGAATTGGAGCCAGAGAAGCGCTCAGGGATCCCT13020.11336772676810553No Hit
GTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGTTGATGGTCAGGGTGG12710.11066849517838873No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12510.10892705544308758No Hit
CCCTCAGTGTCAGTGGCCCCAGGAGAGACGGCCAGGATTGCCTGTGGGGG12100.10535710398572018No Hit
GTTGATGGTCAGGGTGGCCGTGTTCCCAGAATTGGAGCCAGAGAAGCGCT12030.1047476000783648No Hit
GGCCAGGCCCCTGTGCTGGTCATCTCTTCTAATATCGACCGGCCCTCAGG11730.10213544047541305No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGG11690.10178715252835281No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)