FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280738

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280738
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences881949
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT18000.2040934339740733No Hit
CCCCTATCTATGGAACAACACACTACGCACAGAAGTTCCAGGGCAGAGTC14670.16633614868886976No Hit
GTCTGGGGCTGAGGTGAAGAGGCCTGCGTCCTCGGTGAAGGTCTCCTGCA13410.15204960831068465No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA12720.14422602667501183No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12600.14286540378185134No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT12590.14275201854075462No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC10930.1239300685187012No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT10660.1208686670090901No Hit
GAGTTACAGTACCCCTCACACTTTTGGCCAGGGGACCAAGCTGGAGATCA10570.11984819983921972No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC10260.11633325736522179No Hit
TAGTAATACCCTGGCGTCCTCGCACAATAATACACGGCCGTGTCCTCAGA9870.11191123296245022No Hit
ATAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTCATCTCCATGTAGG9630.10918998717612924No Hit
AGATAGGGGTGATCCCTCCCATCCACTCAAGCCCTTGTCCAGGGGCCTGG9500.10771597904187204No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9470.10737582331858192No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA9360.10612858566651814No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9170.1039742660856807No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11200.061.766581
TCAACGC11300.060.5170064
ATCAACG11750.058.1993373
CAACGCA12150.056.8757675
AACGCAG13000.053.1569636
TATCAAC13500.050.121772
CGCAGAG15250.044.3700878
ACGCAGA19100.035.8032577
GTACATG22450.031.7774621
CAGCGCT4400.029.447759
GTACACG7150.028.1892995
ACATGGG23050.027.1694373
TATGTAC8400.026.5652982
GTCCTAC12700.024.3986111
GTATAGC2452.0153293E-623.5301251
GATATAC2151.9551531E-523.4617251
GGCAGCG6800.023.2887427
GTATGTA9600.023.2697031
CATGGGA13400.023.0991484
ACGGGGT8600.022.5994349
Produced by FastQC (version 0.11.5)