FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280739

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280739
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences806353
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA37930.4703895192304115No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT27910.3461263243269387No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20890.25906767879576315No Hit
GTATATTACTGTGCGAAGCTAGCGAGAGATAACCCAATTGACTATTGGGG18400.22818790281675644No Hit
TCTATAAGGCGTCTAATTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGC18170.22533555403154698No Hit
ATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACACCG18160.22521153886697265No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16910.20970964329518213No Hit
GTATAATGGTTTTTCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCA16000.19842426331891863No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15730.1950758538754119No Hit
GTATTAGTACTTGGTTGGCCTGGTATCAGCAGAAACCTGGGAGAGCCCCT15290.18961918663414162No Hit
TCTGGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG14600.18106214027851325No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14410.1787058521516011No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG13700.16990077546682408No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12970.1608476684528984No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12420.1540268344013106No Hit
GTATGTGCTACCATCACTGTTTCTGATAGCTGAGACCCACTCCAGCCCCT12280.15229062209727004No Hit
GCCGTATATTACTGTGCGAAGCTAGCGAGAGATAACCCAATTGACTATTG12270.15216660693269574No Hit
GAGTATTAGTACTTGGTTGGCCTGGTATCAGCAGAAACCTGGGAGAGCCC11840.1468339548559998No Hit
AGCACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGA11550.1432375150833444No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG11490.14249342409589844No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11460.14212137860217547No Hit
CTCCTGTGTAGTCTCTGGACTCACCTTTAGTAGCTATGCCATGAATTGGG11400.14137728761472954No Hit
CCTTCCACCCTGTCTGCGTCTGTAGGAGACAGAGTCACCATCACTTGCCG11390.1412532724501552No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT11180.1386489539940944No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC10760.1334403170819728No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10730.13306827158824983No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGG10670.13232418060080386No Hit
GGACTGAACAGAGAGAATTCACCATGGAGTTTGGGCTGAGCTGGCTGTTT10490.13009190763846604No Hit
CCATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCT10470.12984387730931737No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA10290.12761160434697955No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT10190.1263714527012363No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGTAGTCTCTGGACTCACCTTTA10160.12599940720751335No Hit
CCTTTAGTAGCTATGCCATGAATTGGGTCCGCCAGGCTCCAGGGAAGGGG10020.12426319490347279No Hit
GTACATGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTT9990.12389114940974982No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9900.12277501292858092No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG9780.12128683095368903No Hit
CAGATACACCGTGTTCTTGGAATTGTCTCTGGAGATGGTGAACCGGCCCT9620.11930258832049984No Hit
CCCTGAGACTCTCCTGTGTAGTCTCTGGACTCACCTTTAGTAGCTATGCC9420.11682228502901335No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9410.11669826986443901No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA9350.11595417887699307No Hit
GTGTATCTGCAAATGAACAGCCTGAGAGCCGAGGACACGGCCGTATATTA9340.11583016371241876No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9170.11372190591465525No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9050.11223372393976336No Hit
CACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAAC9020.11186167844604038No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC8930.11074554196487146No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC8930.11074554196487146No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAC8730.10826523867338497No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCGACGACCACGT8570.1062809960401958No Hit
TCTCTGGACTCACCTTTAGTAGCTATGCCATGAATTGGGTCCGCCAGGCT8410.1042967534070066No Hit
ACTTATTACTGCCAACAGTATAATGGTTTTTCTCGGACGTTCGGCCAAGG8360.10367667758413499No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA8330.10330463209041202No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA8170.10132038945722283No Hit
CCTTATAGATCAGGAGTTTAGGGGCTCTCCCAGGTTTCTGCTGATACCAG8090.10032826814062824No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17000.070.024491
TCAACGC19900.059.328334
ATCAACG20800.056.7612423
CAACGCA20950.056.354835
TATCAAC21300.055.13872
AACGCAG22050.053.543486
CGCAGAG25700.045.658948
GCAGAGT28600.041.029199
CACCGAC3750.036.474877
GTGCTAG3600.036.073221
TGCACCG3900.035.0719835
ACCGACC4150.034.693918
GCACCGA3800.034.1004646
GAATACG2750.034.0315749
GTACATG22500.032.3857351
TACATGG22450.032.0946162
CGGGACT900.003755445431.9954954
AGTACGT900.003755445431.9954955
GTGTAGA3700.031.1984621
ACTCGAT950.00489284930.3115239
Produced by FastQC (version 0.11.5)