FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280740

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280740
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences806353
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA32190.39920481476474945No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT23050.2858549543438172No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16640.2063612338516754No Hit
GTATATTACTGTGCGAAGCTAGCGAGAGATAACCCAATTGACTATTGGGG16150.2002844907875335No Hit
ATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACACCG15230.1888750956466957No Hit
TCTATAAGGCGTCTAATTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGC14800.18354244356999974No Hit
GTATAATGGTTTTTCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCA14400.17858183698702676No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14380.17833380665787812No Hit
GTATTAGTACTTGGTTGGCCTGGTATCAGCAGAAACCTGGGAGAGCCCCT13330.16531221437757407No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12960.1607236532883241No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12330.15291069792014167No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10610.13158008961335793No Hit
GTATGTGCTACCATCACTGTTTCTGATAGCTGAGACCCACTCCAGCCCCT10290.12761160434697955No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10170.12612342237208765No Hit
CCATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCT10090.12513130105549305No Hit
GGACTGAACAGAGAGAATTCACCATGGAGTTTGGGCTGAGCTGGCTGTTT9980.12376713424517549No Hit
TCTGGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG9860.1222789522702836No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG9790.12141084611826335No Hit
GCCGTATATTACTGTGCGAAGCTAGCGAGAGATAACCCAATTGACTATTG9790.12141084611826335No Hit
CTCCTGTGTAGTCTCTGGACTCACCTTTAGTAGCTATGCCATGAATTGGG9700.12029470963709442No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT9630.11942660348507414No Hit
GAGTATTAGTACTTGGTTGGCCTGGTATCAGCAGAAACCTGGGAGAGCCC9230.1144659969021012No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA9060.11235773910433769No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT9060.11235773910433769No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9050.11223372393976336No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGG9000.11161364811689174No Hit
AGCACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGA8900.11037349647114848No Hit
GTACATGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTT8710.10801720834423634No Hit
CCTTTAGTAGCTATGCCATGAATTGGGTCCGCCAGGCTCCAGGGAAGGGG8580.10640501120477011No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG8540.10590895054647283No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8430.10454478373615526No Hit
CCTTCCACCCTGTCTGCGTCTGTAGGAGACAGAGTCACCATCACTTGCCG8390.10404872307785795No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC8300.10293258659668904No Hit
GTGTATCTGCAAATGAACAGCCTGAGAGCCGAGGACACGGCCGTATATTA8270.10256054110296609No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG8240.10218849560924309No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGTAGTCTCTGGACTCACCTTTA8170.10132038945722283No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA8090.10032826814062824No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11650.055.6723861
TCAACGC11550.055.468484
ATCAACG12100.052.9471863
CAACGCA12450.050.880525
TATCAAC12950.049.4718862
AACGCAG13550.046.756
CGCAGAG13850.045.217628
GTACATG19950.036.8451651
GAATACG2201.6370905E-1135.9921889
GCAGAGT17200.035.5736739
TACATGG19800.034.5379562
GTGCTAG2950.031.7573721
TAATACC2851.0913936E-1130.309214
CATGGGA12650.029.021374
TATACGG15900.028.9748425
GTATACC1255.3377624E-428.8259221
ATACGGC16050.028.255556
CTAGAGA3850.028.0458584
AATATAC17550.027.4812153
TGCACCG5000.027.3540615
Produced by FastQC (version 0.11.5)