FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280741

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280741
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences429136
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAATAATCAGCCTCATCGTCGGACCGGAGCCCACTGATGGCCAGGGAGG10240.2386189925804407No Hit
GATTATTATTGTGCAGCATGGGATGACAGTCTGAGTGGCCATGTGGTATT9740.22696767458334888No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC9280.21624846202602438No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC9050.21088885574736213No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8530.1987714850303866No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT8300.1934118787517244No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC8080.188285298833004No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA8010.18665411431341114No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT7440.17337161179672644No Hit
CTATAGGAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGGT7340.1710413481973081No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT6760.15752581932068155No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG6690.1558946348010887No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6640.15472950300137953No Hit
GACGTAGGACGGTCAGTTTGGTCCCTCCGCCGAATACCACATGGCCACTC6500.1514671339621938No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6010.1400488423250438No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT5770.13445620968643973No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA5670.13212594608702138No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT5630.13119384064725403No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA5520.1286305506878938No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA5490.1279314716080683No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT5410.1260672607285336No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT5330.12420304984899892No Hit
GCCTTGGGCTGACGTAGGACGGTCAGTTTGGTCCCTCCGCCGAATACCAC5300.12350397076917341No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC4980.11604712725103465No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC4940.1151150218112673No Hit
GTACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCT4750.11068752097237239No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA4680.10905633645277954No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA4670.1088233100928377No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT4660.10859028373289586No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC4630.10789120465307035No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA4430.10323067745423363No Hit
GGTATCAGCAACTCCCAGGAACGGCCCCCAAACTCCTCCTCTATAGGAAT4360.10159949293464077No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)