FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280742

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280742
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences429136
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC11550.2691454457328213No Hit
GATTATTATTGTGCAGCATGGGATGACAGTCTGAGTGGCCATGTGGTATT10010.23325938630177845No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT9200.21438425114648968No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC8030.18712016703329482No Hit
CTATAGGAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGGT7970.1857220088736438No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC7960.18548898251370194No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT7280.16964319003765707No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT6760.15752581932068155No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA6610.154030423921554No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG6400.14913687036277545No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5970.13911673688527645No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC5880.13701949964579993No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC5740.1337571306066142No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT5710.1330580515267887No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA5570.129795682487603No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA5400.12583423436859179No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT5400.12583423436859179No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA5330.12420304984899892No Hit
GACGTAGGACGGTCAGTTTGGTCCCTCCGCCGAATACCACATGGCCACTC5240.12210581260952239No Hit
GTACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCT5220.12163975988963872No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT5060.11791133813056932No Hit
GGTATCAGCAACTCCCAGGAACGGCCCCCAAACTCCTCCTCTATAGGAAT4960.11558107453115098No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT4800.11185265277208158No Hit
GCCTTGGGCTGACGTAGGACGGTCAGTTTGGTCCCTCCGCCGAATACCAC4740.11045449461243055No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC4670.1088233100928377No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG4660.10859028373289586No Hit
GGAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGGTCCAAG4610.10742515193318668No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA4540.10579396741359381No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT4480.10439580925394282No Hit
CCTCTATAGGAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTG4410.10276462473434995No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA4340.10113344021475709No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCC4300.10020133477498974No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10850.066.4976961
TACGTTC350.0042448259.4642875
TATCAAC14350.050.2787442
TCAACGC14750.048.915254
ATCAACG14800.048.7500043
CAACGCA15050.047.479245
AACGCAG15750.045.369056
CTTAGGT804.8394584E-543.3593754
ACGCAGA18650.038.3143427
GTACATG21000.037.991071
CGCAGAG18900.037.807548
TACATGG20550.036.7974432
ACATGGG21350.035.743563
GCAGAGT20400.035.0275739
ATATACG850.00340063232.6470573
GGGACGC1103.1518564E-431.534096
GGTTAGA1552.0627504E-631.3306431
ACTGCGC900.00449861230.8333328
CATGGGG13400.030.0279854
CAGCGCA950.005859836529.2105279
Produced by FastQC (version 0.11.5)