FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280743

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280743
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1190889
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATAGTACTCCCTACACTTTTGGCCAGGGGACCAAGCTGGAGATCA63760.5353983452697942No Hit
GTTTTATACAGCTCCAGTAATAAGAACTACTTAGCTTGGTACCAGCAGAA56800.47695461121901367No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT47140.39583873895887867No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC42880.3600671431174526No Hit
TTATAGTACTCCCTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAAC40460.3397461896112904No Hit
CTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCT39620.3326926355017134No Hit
CTCCTAACCTGCTCATTTATTGGGCATCTACCCGGGAATCCGGGGTCCCT28170.23654597531759888No Hit
GTACTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAG26280.22067547857105071No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25120.21093485622925395No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23580.1980033403616962No Hit
TTATTACTGTCAGCAATATTATAGTACTCCCTACACTTTTGGCCAGGGGA22780.1912856697811467No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG22090.18549167890542276No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT19960.16760588098470974No Hit
CCCTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGC19910.16718602657342538No Hit
GCCTACATGGAGCTGAGCAGCCTGACATCTGAGGACACGGCCGTGTATTA19660.1650867545170037No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18550.15576598658649127No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18460.15501024864617946No Hit
GTTTATTACTGTCAGCAATATTATAGTACTCCCTACACTTTTGGCCAGGG18400.15450642335263823No Hit
ATAGTACTCCCTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA17970.1508956754155929No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC17540.14728492747854754No Hit
ATAATACACGGCCGTGTCCTCAGATGTCAGGCTGCTCAGCTCCATGTAGG16990.14266652895441975No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG16960.14241461630764915No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16880.1417428492495942No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGA16770.14081916954476864No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16200.13603282925612714No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA16040.13468929514001726No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15530.13040678014491694No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA15060.12646014867884414No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15030.1262082360320735No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14940.1254524980917617No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC14870.12486470191596362No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA14850.12469676015144988No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14580.12242954633051442No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA14470.12150586662568888No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC14370.12066615780312018No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG14340.12041424515634959No Hit
GTATTATTGTGCGAGAAAGGGTCCACGCAGTGGTCATTACTTAAAAGACT13940.11705540986607485No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC13810.11596378839673555No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13760.11554393398545121No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13230.11109347722583716No Hit
CCCCTATCTTCGGTACACCAAACTACGCACAGAAGTTCCAGGGCAGAGTC13040.10949803046295667No Hit
CTTCAGTGCCAGCGGGTCTGGGACCGATTTCACTCTCACCATCAGCAGCC12910.10840640899361738No Hit
GTGTAGGGAGTACTATAATATTGCTGACAGTAATAAACTGCCACATCTTC12760.10714684575976435No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12690.10655904958396627No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC12650.10622316605493878No Hit
GGTTAGGAGGCTGCCCTGCTTTCTGCTGGTACCAAGCTAAGTAGTTCTTA12600.10580331164365446No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACT12280.10311624341143465No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG12230.10269638900015031No Hit
GCTCCAGTAATAAGAACTACTTAGCTTGGTACCAGCAGAAAGCAGGGCAG12200.1024444763533797No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC12140.10194065105983847No Hit
GTCCCTGACCGCTTCAGTGCCAGCGGGTCTGGGACCGATTTCACTCTCAC12110.10168873841306789No Hit
CAATAATACACGGCCGTGTCCTCAGATGTCAGGCTGCTCAGCTCCATGTA12110.10168873841306789No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGT12050.10118491311952667No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA11990.10068108782598545No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)