FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280745

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280745
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1194966
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAAAGGCCGTGTCGTCGGCTCTCAGGCTTTTCATTTCCAGAAACA35300.295405894393648No Hit
CTATTACCTGTGGGGGAAAAAACGTTGGAAGTGAAAGTGTGCACTGGTGC33270.2784179633562796No Hit
TATAAAAGGTGTCCAGGGTCAGGTGCAACTGGTGGAGTCTGGGGGAGGCT29630.24795684563410172No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC25560.21389729917001823No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC23410.1959051554604901No Hit
GTGTGAGAGGTGGGCGCACGGAAGTGGGACCCTCGAGCCACTGGGGCCAG22450.18787145408321243No Hit
CCTCTCACACAGTAATAAAAGGCCGTGTCGTCGGCTCTCAGGCTTTTCAT22110.18502618484542654No Hit
GTACGTGCTGTTGTGCTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA20640.1727245796114701No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT20020.1675361474719783No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT19460.1628498216685663No Hit
CCTATGTGCTGACTCAGCCTCCCTCAGTGTCAGTGGCCCCAGGAGAGACG19340.16184560899640657No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA19330.16176192460705996No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGCCTCTG19170.16042297437751368No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC18750.1569082300249547No Hit
CTTTTATTACTGTGTGAGAGGTGGGCGCACGGAAGTGGGACCCTCGAGCC18430.1542303295658621No Hit
TTATTACTGTGTGAGAGGTGGGCGCACGGAAGTGGGACCCTCGAGCCACT17650.147702947196824No Hit
GTACATGGGAGCTCTGGAAGAAGAGCCCCAGCCCCAGAATTCCCAGGAGT17510.146531365745971No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT17500.1464476813566244No Hit
TCGTACAACTGATCATGTGGTGTTCGGCGGCGGGACCAAGCTGACCGTCC16760.14025503654497282No Hit
GTACATGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCA16650.13933450826215976No Hit
CTCCAGGGACAACGCCAAGAACTCAGTGTTTCTGGAAATGAAAAGCCTGA16590.1388324019260799No Hit
TCCCAGGAGTATCCATTCGGTGATCAGCACTGAACACAGAGGACTCACCA16560.13858134875803998No Hit
GTTGTACGATCCCACAGGTGACAATAGTAGTCGGCCTCATCCCCGGCTTC16080.13456449806940116No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG15650.1309660693274955No Hit
AGCCTGGAGGATCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTC15060.1260286903560436No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC14880.12452237134780404No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA14300.11966867676569877No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT14290.11958499237635213No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14260.11933393920831221No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA14080.11782762020007263No Hit
GCCTCTGTGACCTCCTATGTGCTGACTCAGCCTCCCTCAGTGTCAGTGGC14070.117743935810726No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC13980.11699077630660622No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT13630.11406182267947372No Hit
CAGTAATAAAAGGCCGTGTCGTCGGCTCTCAGGCTTTTCATTTCCAGAAA13590.11372708512208715No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC13400.11213708172450096No Hit
GAAGTGGGACCCTCGAGCCACTGGGGCCAGGGAACCCTGGTCACCGTCTC13190.11037970954822145No Hit
CCTCTCTCACTGCACAGCCTCTGTGACCTCCTATGTGCTGACTCAGCCTC13060.10929181248671511No Hit
GACCTAGGACGGTCAGCTTGGTCCCGCCGCCGAACACCACATGATCAGTT12840.10745075592108898No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12720.10644654324892926No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT12510.10468917107264977No Hit
TTTTATTACTGTGTGAGAGGTGGGCGCACGGAAGTGGGACCCTCGAGCCA12430.10401969595787662No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA12420.10393601156853No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG12290.10284811450702364No Hit
GCCTTTTATTACTGTGTGAGAGGTGGGCGCACGGAAGTGGGACCCTCGAG12280.10276443011767698No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTGACTACTTCATGAGCTGGA12240.10242969256029043No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGCAACACGG12050.10083968916270421No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG12010.10050495160531764No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA11990.10033758282662435No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21000.074.8885651
ATCAACG25100.062.289983
TCAACGC25550.061.1928984
CAACGCA26400.059.222675
TATCAAC26950.057.74672
AACGCAG27500.056.5325626
GGTATCA9250.049.133431
CGCAGAG32400.047.9808778
CAGCGCT7650.043.276879
GCAGAGT35950.043.2428479
TACATGG33900.040.8069082
GTACATG34500.040.5658841
ACATGGG34650.039.5077743
GCAGCGC8100.037.3184628
ATGGGAG18050.035.1267245
CATGGGA20500.034.794734
GGCAGCG9000.033.5866137
CATGGGG17550.031.611554
TGGGAGT10600.027.8391276
ACGCAGA57100.027.0995337
Produced by FastQC (version 0.11.5)