FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280746

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280746
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1194966
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAAAGGCCGTGTCGTCGGCTCTCAGGCTTTTCATTTCCAGAAACA30240.2530615933842469No Hit
CTATTACCTGTGGGGGAAAAAACGTTGGAAGTGAAAGTGTGCACTGGTGC26600.22260047566206903No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC26010.21766309669061715No Hit
TATAAAAGGTGTCCAGGGTCAGGTGCAACTGGTGGAGTCTGGGGGAGGCT25990.21749572791192384No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC22350.187034610189746No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT21860.1829340751117605No Hit
GTACGTGCTGTTGTGCTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA20500.17155299816061712No Hit
CCTCTCACACAGTAATAAAAGGCCGTGTCGTCGGCTCTCAGGCTTTTCAT20470.1713019449925772No Hit
GTGTGAGAGGTGGGCGCACGGAAGTGGGACCCTCGAGCCACTGGGGCCAG20320.17004667915237756No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA19020.15916770853731402No Hit
CTCCAGGGACAACGCCAAGAACTCAGTGTTTCTGGAAATGAAAAGCCTGA18020.15079926960264978No Hit
GACCTAGGACGGTCAGCTTGGTCCCGCCGCCGAACACCACATGATCAGTT17990.15054821643460986No Hit
CTTTTATTACTGTGTGAGAGGTGGGCGCACGGAAGTGGGACCCTCGAGCC17290.14469030918034487No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC16880.14125924921713254No Hit
GTACATGGGAGCTCTGGAAGAAGAGCCCCAGCCCCAGAATTCCCAGGAGT16830.14084082727039932No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT16230.13581976390960077No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT16220.13573607952025413No Hit
TCCCAGGAGTATCCATTCGGTGATCAGCACTGAACACAGAGGACTCACCA16120.1348992356267877No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGCCTCTG15870.13280712589312163No Hit
TTATTACTGTGTGAGAGGTGGGCGCACGGAAGTGGGACCCTCGAGCCACT15790.13213765077834852No Hit
TCGTACAACTGATCATGTGGTGTTCGGCGGCGGGACCAAGCTGACCGTCC15580.13038027860206902No Hit
CCTATGTGCTGACTCAGCCTCCCTCAGTGTCAGTGGCCCCAGGAGAGACG15540.13004554104468244No Hit
GTTGTACGATCCCACAGGTGACAATAGTAGTCGGCCTCATCCCCGGCTTC15380.12870659081513616No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCGCCGCCGAACACCAC14530.12159341772067156No Hit
GTACATGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCA14500.12134236455263162No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC14240.11916657042961892No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA14060.11766025142137937No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC13960.11682340752791293No Hit
AGCCTGGAGGATCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTC13760.11514971974098008No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG13730.11489866657294015No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA13460.11263918806058082No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA13460.11263918806058082No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT13330.11155129099907446No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT13250.11088181588430131No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT13050.10920812809736846No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC12760.10678128080631583No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA12520.10477285546199641No Hit
GTATTTGGTGCTTCCACTACTGCTGACATATGAAATCCATTCCAGGCCCT12440.10410338034722327No Hit
TTTTATTACTGTGTGAGAGGTGGGCGCACGGAAGTGGGACCCTCGAGCCA12240.10242969256029043No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTGACTACTTCATGAGCTGGA12180.10192758622421057No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12110.10134179549878407No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT12070.1010070579413975No Hit
GCCTCTGTGACCTCCTATGTGCTGACTCAGCCTCCCTCAGTGTCAGTGGC11950.10000284526923778No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15500.066.404071
TCAACGC16850.059.72474
ATCAACG17000.059.1902733
TATCAAC19100.053.4350472
CAACGCA18800.053.158635
AACGCAG19650.051.242226
GGTATCA7300.049.2989351
CGCAGAG21700.046.0688
GTACATG35900.040.4992751
ACATGGG34850.040.0101553
TACATGG35950.039.7855572
GCAGAGT25650.039.2540939
CAGCGCT7600.038.7984669
GGCAGCG8100.037.292957
ATGGGAG18450.035.851585
GCAGCGC8850.035.7563938
CATGGGA20250.035.497754
CATGGGG18100.030.9771794
TACACGG12150.030.1895336
GTACACG12300.029.8113695
Produced by FastQC (version 0.11.5)