FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280748

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280748
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences278171
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATATTACTGTGCGAAAGATAAAGGGTGGAGGGCATATCCCCCCTCTGA8830.3174306451786851No Hit
GTAATATAGAGCCGTGTCCTCAGGCCACAGGCTAAGCATGTGCAGAAAAA7580.2724942571296073No Hit
GTCTCTATGACCTCCTATGTGCTGACCCAGGCACCCTCAGTGTCAGTGGC4950.1779480966743478No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC4060.14595338838340446No Hit
GCTCTATATTACTGTGCGAAAGATAAAGGGTGGAGGGCATATCCCCCCTC4010.14415593286144135No Hit
TGCTTAGCCTGTGGCCTGAGGACACGGCTCTATATTACTGTGCGAAAGAT3920.14092051292190774No Hit
CCTATGTGCTGACCCAGGCACCCTCAGTGTCAGTGGCCCCAGGAGAGACG3820.13732560187798154No Hit
CTCTATGACCTCCTATGTGCTGACCCAGGCACCCTCAGTGTCAGTGGCCC3740.13444967304284056No Hit
ATATTACTGTGCGAAAGATAAAGGGTGGAGGGCATATCCCCCCTCTGATG3430.12330544880666928No Hit
ATATAGAGCCGTGTCCTCAGGCCACAGGCTAAGCATGTGCAGAAAAAGTG3280.11791308224077994No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA3100.11144224236171275No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT3100.11144224236171275No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCGAAAATCCACTGATCATGA3100.11144224236171275No Hit
GGCTAAGCATGTGCAGAAAAAGTGTGTTCCGGGAATTGTCTCTGGAGACG3000.10784733131778655No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAAAATCCA2950.10604987579582344No Hit
GGCCTGGACCGTTCTCCTCGTCGGCCTCCTCTCTCACTGCACAGTCTCTA2930.10533089358703818No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC2820.10137649143871935No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)