FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280749

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280749
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1027003
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTATATACACTGGGTGCGTCAGGCCCCTGGACAAGGGCTTGAGTGGATG31990.3114888661474212No Hit
CTATATACACTGGGTGCGTCAGGCCCCTGGACAAGGGCTTGAGTGGATGG29490.2871461913937934No Hit
GTCTACATGGAGCTGAGCAGCCTGAGATCTGAAGACGCGGCCGTGTATTA23460.22843165988804315No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC22850.222492047248158No Hit
ATAATACACGGCCGCGTCTTCAGATCTCAGGCTGCTCAGCTCCATGTAGA17700.17234613725568476No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17170.16718549020791565No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATGTAGTCGCCTGCA16290.15861686869463867No Hit
GATTATAACTATGTCTCCTGGTTCCAACAACACCCAGGCAAAGCCCCCAA15960.1554036356271598No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC14660.14274544475527334No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT14390.14011643588188155No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGATTTCCTGCG13630.1327162627567787No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA13340.12989251248535788No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATGTA13300.12950302968929983No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13200.1285293226991547No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC13190.1284319520001402No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC12880.12541346033069037No Hit
CTCCTGGACAGTCAGTCACCATTTCCTGCACTGGAGTCAGCACTGATGTT12350.12025281328292127No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA12120.11801328720558751No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA11770.11460531274007962No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATTTCCTGCACTGGAGTCAG11770.11460531274007962No Hit
GTCAATAAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTC11710.11402108854599256No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA11540.11236578666274587No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC11400.11100259687654272No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT11310.11012626058541213No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG11080.10788673450807837No Hit
GTGTATATAGTAGCTGGTGAAGGTGTATCCAGATGCCTCGCAGGAAATCT10910.10623143262483167No Hit
ATATACACTGGGTGCGTCAGGCCCCTGGACAAGGGCTTGAGTGGATGGGA10760.10477087213961399No Hit
CCATGTAGACTGTGCTCGTGGACGTGTCCCTGGTCATGGTGACTCTGTCA10750.10467350144059949No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG10730.10447876004257048No Hit
CTACTATATACACTGGGTGCGTCAGGCCCCTGGACAAGGGCTTGAGTGGA10620.10340768235341084No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA10530.10253134606228025No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT10500.10223923396523672No Hit
TCTCTGGGCTCCAGGCTGACGATGAGTCTGATTATCACTGCTGCTCATAT10470.10194712186819319No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA10410.10136289767410613No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC10380.10107078557706259No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA10360.10087604417903355No Hit
TCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCT10350.10077867348001904No Hit
TTATAACTATGTCTCCTGGTTCCAACAACACCCAGGCAAAGCCCCCAAAC10300.10029181998494649No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8500.074.687521
GTATCAA25050.065.3736041
ATCAACG25250.063.0615433
TCAACGC25350.062.8127754
CAACGCA25800.061.4379435
TATCAAC26650.060.289452
AACGCAG26850.058.977866
GAATACG3550.050.699
CGCAGAG31600.050.1125148
ACGCAGA34900.045.3740847
GCAGAGT37600.042.6901449
GTGCTAG5050.041.427711
GTACACG7850.035.7955175
ACACGGG8500.033.872857
GTACATG28850.031.75721
ACATGGG29700.031.0518323
ACGGGGT9400.030.62979
GCTAGAG6750.029.8873883
GTGTAGA4500.028.8565441
CTAGAGA7050.028.6155834
Produced by FastQC (version 0.11.5)