FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280752

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280752
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences799412
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA29410.36789540312129415No Hit
GTATATTACTGTGCGAAAGACCCCTACGATGACTACTACTTTGACTTCTG21810.2728255267621702No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT14840.1856364427854473No Hit
ATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGCG12020.1503605149785092No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11710.14648266475859759No Hit
GCCGTATATTACTGTGCGAAAGACCCCTACGATGACTACTACTTTGACTT11620.14535683727539742No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10260.12834433308481735No Hit
ATATTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATT9570.11971298904694952No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9390.1174613340805492No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8910.11145692083681506No Hit
GTACATGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTT8470.1059528753633921No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA8440.10557759953565872No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT8370.10470195593761415No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG8040.10057392183254693No Hit
GTACCACCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA8040.10057392183254693No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA8020.10032373794739134No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT8020.10032373794739134No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC13000.048.1786084
GTATCAA13400.047.8478551
ATCAACG13200.046.9032443
CAACGCA14300.043.7987335
TATCAAC15100.041.9550362
AACGCAG15150.041.341386
CGCAGAG16200.038.661858
GTACATG22750.038.3160931
TACATGG23700.037.362432
TATACGG16300.037.099675
ATACGGC16600.036.8628776
TACGGCC16500.036.6499757
AATATAC18400.033.2567253
TAATATA18950.032.671392
GCAGAGT19450.032.201649
CGTGAGT900.003755130331.996018
ATATACG20200.030.6496454
ACATGGG28650.030.1533093
CATGGGG12400.028.4480654
TCTACAC7950.025.355333
Produced by FastQC (version 0.11.5)