FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280753

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280753
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences393685
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCTAGATAGTGGGGTCCCATCAAGATTCAGTGGCAGTGGATCTGGGAC12400.31497263040247914No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8880.2255610449979044No Hit
GTATAATAGTTATTCTCGGACGTTCGGCCAAGGGACCAAAGTGGAAATCA7370.18720550694082833No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT6030.1531681420424959No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC5870.1491039790695607No Hit
CTATTATACTGTTGGCAGTAATAAGTTGCAAAGTCGTCAGGCTGCAGGTT5420.1376735207081804No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5340.1356414392217128No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT4990.126751082718417No Hit
TCCATACACTGGGTGCGACAGGCTCCTGGAAAAGGGCTTGAGTGGATGGG4860.12344895030290713No Hit
ACCTAATACAGTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAGG4430.11252651231314376No Hit
ATCTAGAATAGATGCCCAATAGATCAGGAGTTTAGGGGCCTTCCCTGGTT4240.10770031878278319No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3980.10109605395176348No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)