FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280756

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280756
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1001139
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACGGCCGTGTCTGCGGCGGTGACAGAGGTCAGGTCCAGGGAGA16700.16681000340612043No Hit
GCATTATGTATCCTGGTACCGCCAGCTCCCAGGAACAGCCCCCCAACTCC16690.16671011717653594No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA14040.14024026633664258No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCC11680.1166671161546998No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT10990.10977496631336908No Hit
GTCTCTGGTGGCTCCATCAGTAGTGACTTCTGGAGCTGGATCCGGCAGCC10680.10667849319624947No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC14200.062.277724
CAACGCA16000.055.2797625
AACGCAG17400.050.8421336
GTATCAA17950.050.5141371
TATCAAC18250.048.8511432
ACGCAGA19150.046.1959847
GGTATCA6250.046.0560651
CGCAGAG20050.044.1223538
GAATACG5050.038.4538969
ATCAACG23850.037.9837723
TAGACGG8900.037.9742245
GCAGAGT25350.035.1812829
AGACGGC9500.034.8258826
ATAGACG9750.034.6584554
GTGCTAG6100.034.2117271
GACGGCC9600.033.7139177
GTAATAG12650.031.288091
GTACATG29750.030.720171
TACATGG29000.029.9987582
AATAGAC12300.028.6422353
Produced by FastQC (version 0.11.5)