FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280757

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280757
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences988674
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT34160.3455132834483358No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30540.30889858537799114No Hit
GTCTACATCTATAGGAGACAGAGTCACCATCACTTGCCAGGCGAGCCAGG29870.30212183186773395No Hit
GTATGACAATCTGCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCA26670.2697552479381474No Hit
ATATTACTGTCAACAGTATGACAATCTGCCCCTCACTTTCGGCGGAGGGA22310.22565577733408584No Hit
ATAGGAGACAGAGTCACCATCACTTGCCAGGCGAGCCAGGGCATTAACAA18490.18701816776814198No Hit
TTGTCATACTGTTGACAGTAATATGTTGCAATATCTTCAGGCTGCAGGCT17980.18185974345436412No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17750.1795333952344251No Hit
ACATATTACTGTCAACAGTATGACAATCTGCCCCTCACTTTCGGCGGAGG17700.1790276673605253No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17290.17488069879454704No Hit
GTAATAAACGGCCGTGTCCTCGGCTCTCAGGCCGTTCAACTGCAGATACA16280.16466499574177132No Hit
ACCTACTACTACCGTGGTTTGGACGTCTGGGGCCAAGGGACCACGGTCAC15770.15950657142799346No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG15390.15566303958635505No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15170.15343783694119598No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG15100.1527298179177363No Hit
TATAAAAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGATCCT13750.139075165322442No Hit
ACTGTCAACAGTATGACAATCTGCCCCTCACTTTCGGCGGAGGGACCAAG13630.13786141842508248No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT13410.1356362157799234No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13040.13189382951306497No Hit
GTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGAGA12980.13128695606438523No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC12940.1308823737652654No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12860.13007320916702572No Hit
GACCAGGGAAAGCCCCTAAACTCCTGATCTACGATGCATCCAATTTGGAA12830.12976977244268587No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12710.12855602554532636No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG12240.12380218353066835No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11740.11874490479167046No Hit
CTATAGGAGACAGAGTCACCATCACTTGCCAGGCGAGCCAGGGCATTAAC11660.1179357401934308No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11620.11753115789431097No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA11600.11732886674475104No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11570.11702543002041117No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA11520.11651970214651139No Hit
CAGTATGACAATCTGCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGAT11230.1135864804778926No Hit
GCCTGAAGATATTGCAACATATTACTGTCAACAGTATGACAATCTGCCCC11200.11328304375355273No Hit
CAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGA10920.11045096765971392No Hit
GTCTCCATCCTCCCTGTCTACATCTATAGGAGACAGAGTCACCATCACTT10850.10974294863625421No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA10820.10943951191191434No Hit
GTCATACTGTTGACAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGC10520.1064051446685156No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT10460.10579827121983586No Hit
GGTATCAGCAGAGACCAGGGAAAGCCCCTAAACTCCTGATCTACGATGCA10360.10478681547203628No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10290.10407879644857658No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC10220.10337077742511687No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC10070.10185359380341752No Hit
GTTTATTACTGTGCGCGAGCAGGATATTGTACTACCACCAGCTGCTCTAC9930.1004375557564981No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21250.074.337841
TCAACGC23550.066.095144
CAACGCA24300.064.055175
ATCAACG24450.063.662193
TATCAAC24850.062.9274332
AACGCAG25750.060.3747986
GTACACG6850.038.924035
CGCAGAG42000.036.8404058
TACACGG7650.034.8112376
ACGCAGA44700.034.7761467
GTACATG28900.034.692991
GCAGAGT46450.033.4659589
ACACGGG8100.032.873957
TACATGG29500.032.7332732
ACATGGG30650.031.7402233
GTCCTAA3000.031.2569681
GTATGTA10200.031.1155321
CATGGGA17750.030.4487574
TATGTAC9400.029.8980882
ACGGGGT9350.029.2487439
Produced by FastQC (version 0.11.5)