FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280760

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280760
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences860232
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCCGCGGCGGTCACAGAGCTCAGGTTCAGGGAGA22320.25946488854169575No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT21740.25272252136632906No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15230.1770452622083345No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC15030.1747203080099322No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14890.1730928400710506No Hit
ACAGTACCCTGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA14520.16879167480400636No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14400.16739670228496498No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA13280.15437695877391214No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC12800.14879706869774667No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11950.1389160133545369No Hit
CCCTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCCTC11700.13600982060653405No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC11030.12822122404188638No Hit
GCATAAACCAGGGACAGCCCCTAAGCTCCTGATCTATGCTGCATCCAATT10890.12659375610300477No Hit
ACCAGGGACAGCCCCTAAGCTCCTGATCTATGCTGCATCCAATTTGCATA10840.1260125175534042No Hit
GGGTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGC10590.12310632480540135No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10540.12252508625580076No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9850.11450399427131286No Hit
GATCTATGCTGCATCCAATTTGCATAGTGGGGTCCCATCAAGGTTCAGTG9820.1141552511415525No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG9540.11090031526378931No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG9320.1083428656455468No Hit
GTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAG9300.10811037022570656No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTACCAA9270.10776162709594621No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT9270.10776162709594621No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA9110.10590166373722439No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT9050.10520417747770369No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT8870.10311171869914162No Hit
TTACAGTACCCTGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAAC8770.10194924159994047No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT8640.10043802137097899No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG8610.10008927824121865No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13100.065.910181
TCAACGC13200.063.7273034
ATCAACG13200.063.7124633
CAACGCA14400.058.416695
AACGCAG15050.055.9099966
TATCAAC15200.055.8021472
CGCAGAG15500.054.28688
GCAGAGT19600.042.930899
ACGCAGA19700.042.7129637
CAGCGCT4600.034.3957639
GGCAGCG5000.033.082477
GTACACG7400.033.0339745
TACATGG21500.030.4239372
TTTCGCG950.004916840230.281449
ACACGGG8100.030.1879757
GTACATG22600.029.9269161
ACATGGG22400.028.5597523
GCAGCGC5950.027.8003968
GTATTGG3153.8198777E-1127.4102651
TACCGTC1050.00801679527.397497
Produced by FastQC (version 0.11.5)