FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280761

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280761
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences793685
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG22940.2890315427405079No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG19830.2498472315843187No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT16700.21041093128886146No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG13980.1761404083484002No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG12800.16127304913158244No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC11920.15018552700378612No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT11580.14590171163622848No Hit
CCCTTAGACTCTCCTGTGCAGGCTCTGGATTCAATTTCAATAGAGCCTGG11300.14237386368647512No Hit
GTGGTACAGTAGTACACGGCTGTGTCCTCGGTTTCCAGGCTGTTCATTTG11100.13985397229379415No Hit
GTAGTACACGGCTGTGTCCTCGGTTTCCAGGCTGTTCATTTGCAGATACA11100.13985397229379415No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10320.13002639586233836No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT10030.12637255334295092No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT9710.12234072711466136No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT8980.1131431235313758No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG8670.10923729187272029No Hit
GTATTACTGTCACCAGTATGGTAACTCACCCCCGTTCTCTTTCGGCGGAG8660.10911129730308625No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)