FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280762

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280762
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences793685
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG20430.25740690576236164No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG18010.2269162199109218No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG13850.17450247894315754No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT11180.14086192885086654No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC10850.13670410805294292No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT10260.12927042844453404No Hit
CCCTTAGACTCTCCTGTGCAGGCTCTGGATTCAATTTCAATAGAGCCTGG9580.12070279770941873No Hit
GTGGTACAGTAGTACACGGCTGTGTCCTCGGTTTCCAGGCTGTTCATTTG9490.11956884658271229No Hit
GTAGTACACGGCTGTGTCCTCGGTTTCCAGGCTGTTCATTTGCAGATACA9290.1170489551900313No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT8820.11112721041723103No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT8430.10621342220150311No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG8390.10570944392296693No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11250.056.387541
ATCAACG11300.054.1456683
TCAACGC11800.052.4613764
CAACGCA13350.045.831165
TATCAAC14450.043.8367232
AACGCAG14250.042.9365656
ACGCAGA14650.042.2555777
CGCAGAG15600.039.6823238
CCGTTGA3550.034.47029
GTCATAG9750.030.3132291
CTACACG950.004895454330.3081634
TAGGTGG9550.030.1494835
CAGAGCG9100.029.267369
GCAGAGT21100.028.6562969
GGTATCA6300.028.6056881
ACCCCGT4550.028.4763536
ATAGGTG9900.027.6294124
CCCGTTG4250.027.0990628
GTACATG20300.026.987991
CATAGGT10250.026.6859683
Produced by FastQC (version 0.11.5)