FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280763

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280763
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences787267
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACATACACTGGGTGCGACAGGCCCCAGGGCAAGGTCTTGAGTGGATGGG33000.41917164062509926No Hit
GTAGTATGTGGCCGTGTCGTCAGATCTCAGGTCCCACAGCACCATGTAGG32560.4135826854167646No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT20040.25455150539778754No Hit
ACATACTACTGTGCGAGGAAGGGACACGATTCTTGGATGACGTCGGACTG19090.2424844430161559No Hit
GTCTGGGGCTGAGGTGGAGAAGCCTGGGGCCTCAGTGAGGGTGTCCTGCA15330.19472428032675065No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14540.1846895652936043No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC13140.1669065259943577No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12940.16436609180875106No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12760.16207970104170505No Hit
GTATGTGGCCGTGTCGTCAGATCTCAGGTCCCACAGCACCATGTAGGCTG12690.16119054907674268No Hit
GCCTACATGGTGCTGTGGGACCTGAGATCTGACGACACGGCCACATACTA12420.1577609629261737No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12110.15382328993848338No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11950.15179094258999806No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT11510.1462019873816634No Hit
GTGTATGTAAAAGCCGTCGAAGTTGTATCCAGAAGCCGTGCAGGACACCC11440.14531283541670106No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG11090.1408670755918894No Hit
CCATGTAGGCTGTCGCACTGGACTTGTCGGTGGTCATGGTAAATCTGCCC10980.13946983678980576No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA10960.1392157933712451No Hit
CAGTAGTATGTGGCCGTGTCGTCAGATCTCAGGTCCCACAGCACCATGTA10750.13654833747635808No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC10390.1319755559422661No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA10100.12829192637313644No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA9920.12600553560609046No Hit
CCTCTAGAGAATCCCCTGAGAACTCCGTTCCTCACCATGGACTGGACCTG9920.12600553560609046No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG9650.12257594945552144No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9570.12155977578127877No Hit
GCCACATACTACTGTGCGAGGAAGGGACACGATTCTTGGATGACGTCGGA9480.12041658039775578No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9450.12003551526991478No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC9430.11978147185135411No Hit
CTTTTGGCCTGGGGACCAAGCTGCAGATCAAACGAACTGTGGCTGCACCA9300.11813018963070979No Hit
ATACTACTGTGCGAGGAAGGGACACGATTCTTGGATGACGTCGGACTGGC8820.11203314758525379No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG8630.10961973510892747No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA8610.10936569169036679No Hit
CATACTACTGTGCGAGGAAGGGACACGATTCTTGGATGACGTCGGACTGG8470.10758738776044215No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT8460.1074603660511618No Hit
GTGCTGTGGGACCTGAGATCTGACGACACGGCCACATACTACTGTGCGAG8190.10403077990059281No Hit
TCCTTGAACAGCGCTCTTCCCACTTGAGGGCGTCCAGGTGAAGGTGACAC8180.10390375819131246No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8160.10364971477275181No Hit
CAGTGAGGGTGTCCTGCACGGCTTCTGGATACAACTTCGACGGCTTTTAC7930.10072821545930415No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)