FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280765

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280765
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences975435
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTACACCCGTCCGGGTAGTGGGAACTACGGTCAAGGACTACTGGGGC30670.31442382116696654No Hit
CCCTTAGACTCTCCTGTGTAGTCTCTGGATTCACTTTCACTAACGCCTGG20760.21282812283750324No Hit
ATATTGCACCCGTGAAAGGTAGATTCACCATCTCAAGCGATGATTCAAAA20030.20534428229456603No Hit
GTAATAGACGGCTGTGTCTTCGGTTTTCAGGCTGTTCATTTGCAGATATA18360.188223715573052No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC17180.17612654866803018No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA16820.17243588757836248No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16560.16977041012471358No Hit
CTCCTGTGTAGTCTCTGGATTCACTTTCACTAACGCCTGGATGACCTGGG16170.16577219394424025No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15640.16033872067334062No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC15480.15869842685571053No Hit
TTACTGTACTACACCCGTCCGGGTAGTGGGAACTACGGTCAAGGACTACT15080.1545976923116353No Hit
GTCTATTACTGTACTACACCCGTCCGGGTAGTGGGAACTACGGTCAAGGA14670.15039443940395825No Hit
GTATTACTGTCAGCAGTATGGTAGCTCACCGTTCACTTTTGGCCAGGGGA14570.1493692557679394No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14530.14895918231353192No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC13920.14270556213381722No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT13770.14116778667978902No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA13230.1356317950452875No Hit
GTGTAGTACAGTAATAGACGGCTGTGTCTTCGGTTTTCAGGCTGTTCATT13090.13419653795486117No Hit
TTTAAAAGGTGTCCAATGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCT12330.12640514232111827No Hit
GATCAGGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGA12210.1251749219578957No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11940.12240692614064494No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG11760.1205615955958111No Hit
GTATATCTGCAAATGAACAGCCTGAAAACCGAAGACACAGCCGTCTATTA11730.12025404050500547No Hit
GTACAGTAATAGACGGCTGTGTCTTCGGTTTTCAGGCTGTTCATTTGCAG10950.11225760814405882No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10700.1096946490540118No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC10600.108669465417993No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10120.10374858396510274No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20050.071.607541
GGTATCA8000.066.7362441
TCAACGC24400.058.148464
ATCAACG24750.057.6171573
CAACGCA25350.055.9635855
AACGCAG26400.053.974556
TATCAAC27100.052.6208342
CGCAGAG30450.046.7932668
GAATACG1454.1472958E-1044.66639
CTATCGG657.610432E-444.284549
ACGCAGA33050.043.1121067
GCAGAGT33250.043.069219
GTACATG26300.030.4499991
GTACACG9800.030.128335
TACACGG10150.029.779066
CAGAGTA35250.028.1725049
TACATGG27200.028.067182
ACATGGG25950.028.0314673
ACACGGG10800.027.9853697
TATGTAC11550.026.1896172
Produced by FastQC (version 0.11.5)