FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280766

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280766
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences975435
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTACACCCGTCCGGGTAGTGGGAACTACGGTCAAGGACTACTGGGGC28780.2950478504462112No Hit
CCCTTAGACTCTCCTGTGTAGTCTCTGGATTCACTTTCACTAACGCCTGG18220.1867884584826257No Hit
ATATTGCACCCGTGAAAGGTAGATTCACCATCTCAAGCGATGATTCAAAA17820.18268772393855048No Hit
GTAATAGACGGCTGTGTCTTCGGTTTTCAGGCTGTTCATTTGCAGATATA17020.17448625485040006No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16520.16936033667030606No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC15690.16085131249135No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15130.15511028412964473No Hit
CTCCTGTGTAGTCTCTGGATTCACTTTCACTAACGCCTGGATGACCTGGG14870.15244480667599583No Hit
GTGTAGTACAGTAATAGACGGCTGTGTCTTCGGTTTTCAGGCTGTTCATT14350.14711385176869807No Hit
GTCTATTACTGTACTACACCCGTCCGGGTAGTGGGAACTACGGTCAAGGA14330.1469088150414943No Hit
GTATTACTGTCAGCAGTATGGTAGCTCACCGTTCACTTTTGGCCAGGGGA14310.14670377831429054No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14090.14444837431504917No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC13500.13839979086253826No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA13360.13696453377211193No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT13080.1340940195912593No Hit
TTACTGTACTACACCCGTCCGGGTAGTGGGAACTACGGTCAAGGACTACT12690.13009580341078597No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC12190.12496988523069195No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11320.11605078759732838No Hit
TTTAAAAGGTGTCCAATGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCT11190.11471804887050394No Hit
GTATATCTGCAAATGAACAGCCTGAAAACCGAAGACACAGCCGTCTATTA11010.11287271832567007No Hit
GATCAGGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGA10490.10754176341837231No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG10420.10682413487315914No Hit
GTACAGTAATAGACGGCTGTGTCTTCGGTTTTCAGGCTGTTCATTTGCAG10220.10477376760112155No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA10190.10446621251031592No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10070.10323599214709335No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG13900.062.559633
GTATCAA14200.061.8424381
TCAACGC14350.060.6071624
CAACGCA15200.057.226765
AACGCAG15250.057.047926
TATCAAC16950.051.726582
CGCAGAG16900.051.052718
GGTATCA6350.044.2085231
ACGCAGA20350.042.7508967
GCAGAGT21050.041.3292549
GAATACG1951.8735591E-1036.8714039
CAGCGCT5800.034.7099769
GTACATG26650.033.221791
TACATGG25500.032.9738242
TAGCGCG1102.5626714E-432.6714024
ACATGGG26950.031.7330513
GTAGCGC1602.0102125E-631.4413853
GTACACG9050.030.1850835
GGCAGCG7700.028.9464457
GTATGTA11550.028.6675761
Produced by FastQC (version 0.11.5)