FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280767

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280767
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1428986
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT47350.33135384111530836No Hit
ATATAAAACTTACTGGACGTTCGGCCAAGGGACCAAGGTGGAAATGAGAC46900.32820475498010476No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41490.2903457416657686No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG34910.24429910439990316No Hit
TCTCTAAGGTGTCTAGTCTAGAAAGTGGGGTCCCATCCAGGTTCAGTGGC32210.22540458758868176No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGAATTGACAC29700.20783968492343521No Hit
GTTTTATATTGTTGGCAGTAATAAGTTGCAAAATCATCCGGCTGCAGGCT29320.20518045663148557No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29100.2036409034098305No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG27980.19580317791776827No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27930.19545327945830118No Hit
GCATGGACATGGGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG25750.18019770662553727No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT25410.17781839710116126No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG25310.1771186001822271No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG24250.1697007528415254No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG23270.16284274303597096No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA23170.16214294611703683No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG23020.16109325073863565No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22750.1592037990575135No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21060.1473772311275268No Hit
CTTTTATACTGGCAACACCTTCTACAACCCGTCCCTCAAGAATCGAGTCA20850.14590765759776514No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT19160.1340810896677784No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19070.13345127244073768No Hit
ACTTATTACTGCCAACAATATAAAACTTACTGGACGTTCGGCCAAGGGAC19060.13338129274884428No Hit
CACCATCACCAGCCTGCAGCCGGATGATTTTGCAACTTATTACTGCCAAC18530.12967236907849344No Hit
AGTCAGGACACAGCATGGACATGGGGGTCCCCGCTCAGCTCCTGGGGCTC18460.12918251123523952No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC18450.12911253154334612No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT18110.1267332220189701No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG17890.12519366879731503No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA17560.1228843389648324No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC17320.1212048263593905No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT17250.12071496851613663No Hit
CCTTAGAGATCAAGACCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAG17070.11945533406205519No Hit
GACATGGGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCC16840.11784580114850671No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA16810.11763586207282647No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAAGGTCTTGATCTCTAAGGTG16430.11497663378087679No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16430.11497663378087679No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC16100.11266730394839418No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG15800.11056791319159179No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15460.10818860366721578No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15380.10762876613206848No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA15000.1049695378401188No Hit
CAGCATGGACATGGGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCT14990.1048995581482254No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC14880.10412978153739785No Hit
GTAATAAGTTGCAAAATCATCCGGCTGCAGGCTGGTGATGGTGAGAGTGA14580.10203039078059548No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28750.063.2363241
TCAACGC30950.058.652864
ATCAACG31350.057.4449423
CAACGCA32550.055.985195
TATCAAC32950.054.6555022
AACGCAG34250.052.951566
ACGCAGA37350.048.360587
CGCAGAG38050.047.2817738
GCAGAGT43100.042.2426959
GTACATG40250.039.0745961
TACATGG41000.037.7749522
TACGCTA2104.0927262E-1034.268039
GTCATAG16650.034.2307781
ATACGCT2155.275069E-1033.4710968
CATGGGA23700.031.9146634
CAGAGTA42650.031.0461089
GATTCAC5900.030.569751
ATAGGTG17250.030.4847834
CATAGGT18450.030.4542243
TAGGTGG17550.030.3709435
Produced by FastQC (version 0.11.5)