FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280769

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280769
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences903543
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT12490.1382335981796107No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11520.12749808254836792No Hit
GTAATACACGGCCGTGTCCTCGGATCTCAGGCTGCTCAGGTCCATGTAGG9890.10945798927112489No Hit
TTATTAAACTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCT9590.10613772670476115No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9200.10182138536848828No Hit
GTTTAATAATTACCCTCTTTTCGGCGGAGGGACCAAGGTGGAGATCAAAC9190.10171070994960948No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15150.068.061911
ATCAACG18600.054.237463
TCAACGC19100.052.7883834
CAACGCA20350.049.5458535
AACGCAG21200.047.543556
TATCAAC21600.047.043542
ACGCAGA25700.039.4969527
CGCAGAG26100.038.8916328
GCAGAGT30250.033.7830479
CAGAGTA28150.030.2104199
GGTATCA16500.026.90681
GTACATG24750.026.328161
TTAGGCG1500.001618993923.8358924
ACATGGG24750.023.7045573
GTATGTA7250.023.7043231
CTCTATA3707.728886E-921.2743532
TATACTA5051.8189894E-1221.2399025
GTACACG5101.8189894E-1221.0316685
TATGTAC6250.020.6090452
TATATAC4551.8371793E-1020.4420833
Produced by FastQC (version 0.11.5)