FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280770

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280770
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences903543
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT10790.11941877697021613No Hit
GTTTAATAATTACCCTCTTTTCGGCGGAGGGACCAAGGTGGAGATCAAAC10380.11488108479618568No Hit
GTAATACACGGCCGTGTCCTCGGATCTCAGGCTGCTCAGGTCCATGTAGG9430.10436692000270048No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10900.055.5004651
TCAACGC10900.053.5183074
ATCAACG11700.049.8589363
CAACGCA12450.047.4338465
AACGCAG13350.044.2213676
TATCAAC13650.043.263842
CGCAGAG16150.036.554518
ACGCAGA17000.034.726787
GTACACG4850.034.1530845
GTACATG23700.030.0836621
ACATGGG24250.029.104373
ACACGGG5850.028.305557
GCAGAGT21300.028.0542139
GTCTAAC1804.9493447E-628.0071811
GTATAAC3153.8198777E-1127.4356041
TACCGTT1050.00797573427.4264977
GTATGTA7650.026.35971
TATCGTG1658.827883E-526.1798367
GTATAGA5250.026.0638241
GTATAAA5450.025.1073531
Produced by FastQC (version 0.11.5)