FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences714319
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCAGATCTCAGGCTGTTCATTTGCAGAGACA32890.46043854356386993No Hit
GTGTATTACTGTGCGACAAGAGGATTACTACCTGCGAACGCTGAATACTT28640.40094131613466816No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC19890.2784470243686644No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGCTGGAGTCCGGGGGAGGCG19050.26668757235912804No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATACCACAGAATTACTC18440.2581479703045838No Hit
GATGTACACTGGTACCAACACCTTCCAGGAACAGCCCCCAAACTCCTCAT17880.2503083356315596No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA16160.22622945770727085No Hit
TCCAGGAACAGCCCCCAAACTCCTCATCTATGGAAACACCAATCGGCCCT16040.2245495359916228No Hit
GCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGC15810.2213296860366307No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15260.21363004483991047No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACT15150.21209011660056643No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT14910.20873027316927031No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC14690.20565041669058223No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT14670.20537042973797423No Hit
GGTTATGATGTACACTGGTACCAACACCTTCCAGGAACAGCCCCCAAACT14490.20285054716450215No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATACCAC14280.19991068416211805No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA13720.19207104948909381No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC12460.17443187147478928No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG12060.1688321324226291No Hit
GTATTACTGTGCGACAAGAGGATTACTACCTGCGAACGCTGAATACTTCC11840.165752275943941No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT11670.16337238684677294No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA11610.1625324259889489No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG11530.16141247817851687No Hit
CTCCAGAGACAGTTCCCAGAACACGCTGTCTCTGCAAATGAACAGCCTGA11110.1555327521737487No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG10960.15343285002918863No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10540.14755312402442047No Hit
GTTACTATGCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAG10410.1457332088324684No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC10410.1457332088324684No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATACCACAGAATTACTCG10350.14489324797464437No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9840.13775358068314017No Hit
ATGCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTG9820.13747359373053217No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT9660.1352336981096681No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA9600.13439373725184406No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC9470.13257382205989202No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT9420.13187385467837198No Hit
CTTCCAGCACTGGGGCCAGGGCACCCTGGTCACCGTCACCTCAACCTCCA9410.13173386120206798No Hit
CTCCTGTGCAGCCTCGGGATTCACCTTCAGTTACTATGCTATGCACTGGG9360.13103389382054795No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG9330.13061391339163594No Hit
TTACTATGCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGT9150.12809403081816387No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA9030.12641410910251583No Hit
GTGCTGGAAGTATTCAGCGTTCGCAGGTAGTAATCCTCTTGTCGCACAGT8970.1255741482446918No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA8910.12473418738686776No Hit
GTACATGGGAGCTCTGGGAGACGAGCCCAGCACTGGAAGTCGCCGGTGTT8800.12319425914752373No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC8790.12305426567121973No Hit
CAGTAATACACAGCCGTGTCCTCAGATCTCAGGCTGTTCATTTGCAGAGA8770.12277427871861171No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA8760.12263428524230771No Hit
GCTGTGTATTACTGTGCGACAAGAGGATTACTACCTGCGAACGCTGAATA8700.12179432438448369No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA8660.12123435047926767No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA8650.12109435700296367No Hit
ATACACAGCCGTGTCCTCAGATCTCAGGCTGTTCATTTGCAGAGACAGCG8550.1196944222399236No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG8550.1196944222399236No Hit
GAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTCGT8530.11941443528731562No Hit
GGGCTGGAGTGGGTGGCAGTTATATCATATGATGGAAGTGAGAAATACTA8400.11759452009536356No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT8310.11633457880862752No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGGACTGGCT8260.1156346114271075No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG8250.1154946179508035No Hit
GATCAGCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGG8080.11311472885363542No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT8080.11311472885363542No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGG8060.11283474190102742No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG7980.11171479409059538No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA7970.11157480061429138No Hit
GATTATTACTGCCAGTCCTATGACACCAGCCCGAGTAATTCTGTGGTATT7920.11087483323277134No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC7900.11059484628016335No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG7830.10961489194603533No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG7790.10905491804081931No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA7750.10849494413560329No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC7630.10681502241995523No Hit
GTCTCTGCAAATGAACAGCCTGAGATCTGAGGACACGGCTGTGTATTACT7570.10597506156213121No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC7530.1054150876569152No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA7490.10485511375169917No Hit
ATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAGTT7480.10471512027539517No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT7410.10373516594126714No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC7230.10121528336779506No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT7230.10121528336779506No Hit
TCTATGGAAACACCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGC7210.10093529641518706No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4250.082.537861
GTATCAA14150.080.442711
TATCAAC16650.067.9342652
TCAACGC16800.067.2710954
ATCAACG16900.066.901173
CAACGCA17300.065.326845
AACGCAG17700.063.832646
CGCAGAG20550.054.631978
GCAGAGT23950.047.1418349
GCAGCGC4000.035.754368
CAGCGCT4100.034.8578879
AATACAC17550.033.0271573
GTAATAC17400.032.9144631
CAGCCGT17200.032.8211339
TACATGG18100.032.0370452
GTACATG18500.031.7313211
TAGACTG1602.0843254E-631.2938355
GGTAAGG1153.4045085E-431.1256312
ACATGGG18650.031.0791783
TATTGAG7850.030.9806145
Produced by FastQC (version 0.11.5)