FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280772

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280772
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences714319
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCAGATCTCAGGCTGTTCATTTGCAGAGACA31810.44531924812303747No Hit
GTGTATTACTGTGCGACAAGAGGATTACTACCTGCGAACGCTGAATACTT26490.3708427187293072No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC18740.26234777459370395No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGCTGGAGTCCGGGGGAGGCG17820.2494683747737355No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17250.2414887466244073No Hit
GATGTACACTGGTACCAACACCTTCCAGGAACAGCCCCCAAACTCCTCAT15310.2143300122214305No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATACCACAGAATTACTC15180.21251009702947843No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14970.20957023402709435No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT14500.20299054064080616No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACT14410.20173059935407012No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCC14050.19669083420712596No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14030.1964108472545179No Hit
GGTTATGATGTACACTGGTACCAACACCTTCCAGGAACAGCCCCCAAACT13860.19403095815734986No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA13810.19333099077582985No Hit
GCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGC13750.19249102991800582No Hit
TCCAGGAACAGCCCCCAAACTCCTCATCTATGGAAACACCAATCGGCCCT13710.1919310560127898No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC12650.17709174752456536No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC12160.17023206718566913No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATACCAC11870.16617225637285302No Hit
GTATTACTGTGCGACAAGAGGATTACTACCTGCGAACGCTGAATACTTCC11040.15455279783962067No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG10890.1524528956950606No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC10670.14937303921637252No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT10430.1460131957850764No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG10270.14377330016421236No Hit
CTCCAGAGACAGTTCCCAGAACACGCTGTCTCTGCAAATGAACAGCCTGA9930.1390135219698762No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA9760.13663363287270813No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG9670.1353736915859721No Hit
GTCCTGGTGCACAACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT9540.13355377639402002No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC9530.13341378291771602No Hit
CTCCTGTGCAGCCTCGGGATTCACCTTCAGTTACTATGCTATGCACTGGG9470.13257382205989202No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA9460.13243382858358801No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC9280.12991394601011594No Hit
GTGCTGGAAGTATTCAGCGTTCGCAGGTAGTAATCCTCTTGTCGCACAGT8840.12375423305273975No Hit
CAGTAATACACAGCCGTGTCCTCAGATCTCAGGCTGTTCATTTGCAGAGA8770.12277427871861171No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT8720.12207431133709169No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG8660.12123435047926767No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT8610.12053438309774765No Hit
GTTACTATGCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAG8360.11703454619014754No Hit
TTACTATGCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGT8300.11619458533232352No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT8220.11507463752189148No Hit
CTTCCAGCACTGGGGCCAGGGCACCCTGGTCACCGTCACCTCAACCTCCA8170.11437467014037146No Hit
ATGCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTG8170.11437467014037146No Hit
ATACACAGCCGTGTCCTCAGATCTCAGGCTGTTCATTTGCAGAGACAGCG8160.11423467666406746No Hit
GTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC8080.11311472885363542No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATACCACAGAATTACTCG8080.11311472885363542No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA8040.1125547549484194No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT7970.11157480061429138No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGT7920.11087483323277134No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG7880.11031485932755535No Hit
GAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTCGT7820.10947489846973131No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG7800.10919491151712331No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG7740.10835495065929929No Hit
GATTATTACTGCCAGTCCTATGACACCAGCCCGAGTAATTCTGTGGTATT7590.10625504851473921No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT7520.10527509418061119No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA7510.10513510070430718No Hit
GGGCTGGAGTGGGTGGCAGTTATATCATATGATGGAAGTGAGAAATACTA7460.10443513332278716No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA7450.10429513984648316No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA7410.10373516594126714No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC7410.10373516594126714No Hit
GCTGTGTATTACTGTGCGACAAGAGGATTACTACCTGCGAACGCTGAATA7300.1021952377019231No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC7290.1020552442256191No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACAACGGTGAGGACGC7270.10177525727301108No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT7260.10163526379670708No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT7200.10079530293888306No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3000.076.7953341
TCGTATG502.0804122E-457.59652
GTATCAA8500.047.432411
TCAACGC7900.047.3895233
CAACGCA8450.044.3054
TACACCG657.5917685E-444.3049965
ATCAACG8400.043.7116282
AACGCAG8600.042.695085
TATCAAC8900.042.064862
CGCAGAG9250.038.1328857
CTTAGAC1001.4493587E-435.9978143
TACATGG14850.035.391792
GTACATG14750.035.1436271
ACATGGG14850.033.9373323
CATGGGA6650.031.3965874
GCAGAGT12050.030.4669138
CAGAGTA11950.030.1194749
CTATAAT2401.7025741E-929.9981751
GTAATAC18000.029.59821
CAGCCGT18200.029.2688489
Produced by FastQC (version 0.11.5)