FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280774

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280774
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences871841
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC23120.2651859685424292No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG21390.24534290082710036No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCCATGATCACTA18010.20657436390350992No Hit
CCCCATGATCACTACTACTATCCCACACCTGACAGTAATAGTCGGCCTCA16410.18822239376216535No Hit
GTAGTAGTGATCATGGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTA16000.18351970141344578No Hit
CCTATGTGCTGACTCAGCCACCCTCAGTGTCAGTGGCCCCAGGAAAGACG15900.18237270327961178No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA15320.17572011410337435No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCC14850.17032922287435437No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC14680.16837932604683653No Hit
TTATGATAGTGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCA14500.16631472940593525No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT14090.16161203705721572No Hit
CTACTACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTC13590.15587704638804553No Hit
TAGTAGTAGTGATCATGGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCC13550.1554182471345119No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA13540.15530354732112853No Hit
ATCATGGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC12900.14796275926459068No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA12630.14486586430323878No Hit
GTACATGGGAGTCTGGGCCTAAGGAAGCAGCACAGGTGGTGCCTCAGCCA12460.1429159674757209No Hit
CACTACTACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCT12330.14142486990173667No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT12190.139819072514369No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG11630.1333958829648984No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC11510.13201948520429757No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11450.13133128632399715No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT11440.13121658651061374No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA11160.12800499173587845No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA11150.12789029192249504No Hit
GTAATACATGGCGGTGTCCGAGGCCTTCAGGCTGCTCCATTGCAGATAGG11080.1270873932288112No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCAC11040.1266285939752776No Hit
CCCCTGTGCTGGTCATCTATTATGATAGTGACCGGCCCTCAGGGATCCCT11030.1265138941618942No Hit
ATGTATTACTGTGCAAGACCGGGGGATAGTAGTGAAAGCTCCAACTTTGA11000.126169794721744No Hit
GGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCAGTGTCAGTGGC10890.12490809677452655No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGG10790.12376109864069253No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG10750.1233022993871589No Hit
GTGCTGGTCATCTATTATGATAGTGACCGGCCCTCAGGGATCCCTGAGCG10580.12135240255964103No Hit
CTATCATAATAGATGACCAGCACAGGGGCCTGGCCTGGCTTCTGCTGGAA10250.11756730871798872No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG9750.11183231804881855No Hit
GCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCAGTGTCA9510.10907952252761685No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT9490.10885012290085005No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT9420.1080472242071662No Hit
GTGCTGACTCAGCCACCCTCAGTGTCAGTGGCCCCAGGAAAGACGGCCAC9260.10621202719303176No Hit
CTACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGAC9250.10609732737964836No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9150.10495032924581432No Hit
ATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCCGA9110.1044915299922807No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCCATGATCACTAC9070.1040327307387471No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG9010.10334453185844665No Hit
GCACAGGGGCCTGGCCTGGCTTCTGCTGGAACCAGTGCACAATTTTACTT8980.10300043241829646No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC8830.1012799352175454No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT8740.10024763689709477No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10300.062.2223171
TCAACGC10050.059.4710244
ATCAACG10800.056.0078543
TATCAAC11800.051.2614252
CAACGCA11750.050.866715
AACGCAG12900.046.3187946
CGCAGAG14300.040.777248
GGTATCA4750.039.4160541
GTACATG22150.036.7365261
ACATGGG22100.036.167973
ACGCAGA16550.035.2335057
CATGGGA11800.033.5640264
TACATGG28300.031.0432222
GTACACG5200.030.465815
GCAGAGT19500.030.2724889
TACACGG5500.030.1046776
ACACGGG5450.029.0599587
GACCGTT16550.027.4038377
ACCGTTC16750.027.076638
TATGTAC6400.027.0037882
Produced by FastQC (version 0.11.5)