FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280776

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280776
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1072723
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTAGG24820.23137380292955403No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC23060.2149669579192392No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA20420.19035669040376688No Hit
GTGTATTACTGTGCACGATCGGGAAGTTATTACTATGATAGTAGTGGTTA18070.16844982348658508No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17960.1674243956734404No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGCATAACTG17390.16211081518714524No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15880.14803448793397736No Hit
GCCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTA15860.1478480465134056No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTT15740.14672939798997506No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA15540.14486498378425744No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT15320.1428141281579681No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG14980.13964462400824817No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA14800.13796665122310234No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14650.13656834056881412No Hit
GATTATTACTGCTGCTCATATGCAGGCAGTTATGCTTGGGTGTTCGGCGG14620.1362886784379565No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC14500.13517002991452592No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA14260.1329327328676648No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC14210.13246662931623543No Hit
GGTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAA13780.12845813877394258No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC13220.1232377789979333No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTATAACTATGTCTCCT12360.11522079791334763No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA12320.1148479150722041No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT12290.11456825294134645No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT12140.11316994228705826No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAG12090.11270383873562886No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA11990.11177163163277007No Hit
GTATTACTGTGCACGATCGGGAAGTTATTACTATGATAGTAGTGGTTATA11410.10636483043618902No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCTGCTCATAT11260.10496651978190083No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG10960.10216989847332443No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC10920.1017970156321809No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC12500.069.1264
GTATCAA12800.068.0684051
ATCAACG13000.066.46733
CAACGCA13400.063.9458475
AACGCAG14350.060.1946376
TATCAAC14850.058.1868632
CGCAGAG17150.049.9472168
GGTATCA5400.042.670371
ACGCAGA20150.042.5109067
GTACATG25900.039.2003141
TAGCGCG750.001534091738.403334
CAGCGCT5950.036.2938239
TATACCG1001.4485858E-436.0031245
ACATGGG27400.034.9519353
TATTGAG13800.032.350635
CCGTCCG900.003757810231.9923349
CATGGGG18800.029.4919194
GCAGCGC8550.029.4666238
GTGCTAG3301.8189894E-1228.3660981
GGCAGCG7750.027.864297
Produced by FastQC (version 0.11.5)