FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280777

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280777
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences778363
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATACA26840.3448262571576501No Hit
GTTTATTACTGTGCGAATATAGGATATTGTACTGGTGGTGTGTGCTCTGA22030.28302989736151385No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT20750.26658512801867507No Hit
ATATTATTGTCAGCAGTATGCCACTCTCCCTCCGTGGACGTTCGGCCAAG18200.23382406409348852No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16800.21583759762475863No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG16500.2119833548100308No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT14520.1865453522328271No Hit
CTATTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCT14400.18500365510693595No Hit
GACCAGGGAAAGCCCCTAAGCTCCTGATCTACGATGCATCCAATTTGGAA14360.18448975606497225No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13570.17434024998618897No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA13170.16920125956655185No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC12750.1638053196259329No Hit
GTATAAGCACAGACCAGGGAAAGCCCCTAAGCTCCTGATCTACGATGCAT12530.1609788748951325No Hit
CCGTAATAGTGATAGGGGGAGTGGTGGTCAGAGCACACACCACCAGTACA12400.1593087030087504No Hit
ATATTGTACTGGTGGTGTGTGCTCTGACCACCACTCCCCCTATCACTATT12100.1554544601940226No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA11870.15249954070273125No Hit
CAATAATATGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGGTGAGTAT11830.15198564166076756No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11740.15082936881634917No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11460.14723207552260323No Hit
GTACAATATCCTATATTCGCACAGTAATAAACAGCCGTGTCCTCAGCTCT11430.14684665124113042No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11000.14132223654002052No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC10870.13965206465363847No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10730.13785341800676548No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10390.13348527615007394No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC10240.13155815474271004No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10170.13065883141927354No Hit
TCCCTGGTCTGTGCTTATACCAATTTAAATAGTTGTAAATGTCCTGACTC10000.12847476049092776No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9920.12744696240700032No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9860.12667611384405475No Hit
GTACTGGTGGTGTGTGCTCTGACCACCACTCCCCCTATCACTATTACGGT9440.12128017390343579No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG9220.11845372917263539No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG9150.11755440584919889No Hit
GTATAAAATCTTTCCCAGATCCTCTTCCACTGAACCTTGATGGGACCCCT8830.11344321351348921No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG8720.11202999114808901No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT8590.11035981926170693No Hit
GGTATAAGCACAGACCAGGGAAAGCCCCTAAGCTCCTGATCTACGATGCA8540.1097174454592523No Hit
CCCTATCACTATTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCAC8170.10496387932108797No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG8090.10393608123716055No Hit
GTATGCCACTCTCCCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAA8040.10329370743470592No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG7920.10175201030881478No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT7820.1004672627039055No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATACG2300.043.8246049
CAGCGCT4200.041.1414649
ATCAACG22800.040.422283
TCAACGC22700.040.283164
CAACGCA22900.039.9313435
TATCAAC24150.037.8645022
GGCAGCG4600.037.5639467
GTATCAA27750.036.3511161
CGCAGAG25800.035.440668
GTGCTAG2850.035.3945051
GCAGCGC5150.033.552268
GCAGAGT28150.032.737799
GCTAGAG3600.032.0009733
CTAGAGA4550.030.0668454
TACATGG24500.028.2131022
GTACATG24950.027.7238391
ACATGGG25400.027.4968993
GTGTAGA3505.456968E-1226.7625791
TGCTAGA4850.026.7224622
CTGTGCG14400.026.4991049
Produced by FastQC (version 0.11.5)