FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280778

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280778
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences778363
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATACA26280.33763167057015814No Hit
GTTTATTACTGTGCGAATATAGGATATTGTACTGGTGGTGTGTGCTCTGA22380.28752651397869633No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT21030.2701824213124211No Hit
ATATTATTGTCAGCAGTATGCCACTCTCCCTCCGTGGACGTTCGGCCAAG18500.23767830690821634No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17270.22187591136783222No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG15590.20029215160535635No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15430.19823655543750154No Hit
GACCAGGGAAAGCCCCTAAGCTCCTGATCTACGATGCATCCAATTTGGAA13360.1716422800158795No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13160.16907278480606094No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA13090.1681734614826244No Hit
CAATAATATGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGGTGAGTAT12420.1595656525297323No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12420.1595656525297323No Hit
CTATTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCT12300.15802395540384112No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC12100.1554544601940226No Hit
GTATAAGCACAGACCAGGGAAAGCCCCTAAGCTCCTGATCTACGATGCAT11770.15121479309782196No Hit
CCGTAATAGTGATAGGGGGAGTGGTGGTCAGAGCACACACCACCAGTACA11690.15018699501389454No Hit
GTACAATATCCTATATTCGCACAGTAATAAACAGCCGTGTCCTCAGCTCT11660.14980157073242176No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11540.14825987360653062No Hit
ATATTGTACTGGTGGTGTGTGCTCTGACCACCACTCCCCCTATCACTATT11050.14196461034247518No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11020.1415791860610024No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10920.1402944384560931No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC10520.135155448036456No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10490.1347700237549832No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC10480.1346415489944923No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG9960.12796086144896404No Hit
GTATAAAATCTTTCCCAGATCCTCTTCCACTGAACCTTGATGGGACCCCT9540.12256492150834507No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9420.12102322438245394No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG9370.12038085057999931No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT9240.11871067869361725No Hit
GTACTGGTGGTGTGTGCTCTGACCACCACTCCCCCTATCACTATTACGGT8980.11537033492085313No Hit
GTATGCCACTCTCCCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAA8700.11177304162710713No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA8680.11151609210612529No Hit
TCCCTGGTCTGTGCTTATACCAATTTAAATAGTTGTAAATGTCCTGACTC8660.11125914258514344No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG8530.10958897069876138No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG8450.10856117261483396No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT8250.1059916774050154No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT8240.10586320264452448No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT8060.10355065695568777No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT8000.1027798083927422No Hit
GTGATAGGGGGAGTGGTGGTCAGAGCACACACCACCAGTACAATATCCTA7940.10200895982979663No Hit
GGTATAAGCACAGACCAGGGAAAGCCCCTAAGCTCCTGATCTACGATGCA7900.10149506078783292No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAA400.006216143754.0047455
GTATACG657.574631E-444.325831
GAATACG2250.038.3959739
GGCAGCG3600.035.9985357
ATCAACG13850.034.3178943
TCAACGC13900.034.190064
GCAGCGC4150.032.9625178
CAACGCA14600.032.5508045
CAGCGCT4350.031.4449779
TATCAAC15450.031.2320672
CGCAGAG15850.030.4340958
GTGCTAG2901.2732926E-1129.80531
TACACGG6400.028.123866
CTGTGCG13850.028.0692589
GTACACG6500.027.6947425
GTACATG22700.026.654081
TACATGG21900.026.6376132
ATAAGAC2456.866503E-826.4547063
GCAGAGT18400.026.2146429
ACACGGG7000.025.7132427
Produced by FastQC (version 0.11.5)