FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280780

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280780
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences682918
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCAACTGCAGATACA36800.5388641096002741No Hit
GTATACTACTGTGCGGCCAACCGGCTGCCATGGGCTCTTGATATCTGGGG24570.35977965143692214No Hit
GTATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCAACTGCAGATACAGCG19630.28744300194166794No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17210.2520068295168673No Hit
GCCGTATACTACTGTGCGGCCAACCGGCTGCCATGGGCTCTTGATATCTG13420.1965096834466217No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA13280.19445965694270761No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA12990.19021317347031413No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12640.18508810721052893No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC12300.18010947141530903No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC12290.17996304095074372No Hit
ATTTATAACGGTGCTAGTAGCACCTACTATGCAGACTCCGTGAAGGGCCG12030.17615584887204613No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC11370.16649143821073686No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10850.15887705405334168No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG10770.15770561033681937No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10070.1474554778172489No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT9840.1440875771322472No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA9670.14159825923463726No Hit
GTACATGGGAGCTCCGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTT9580.14028038505354962No Hit
GCATAGTAGGTGCTACTAGCACCGTTATAAATAAGTGAGACCCACTCCAG9550.13984109365985375No Hit
CTACTATGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGATAATT9450.13837678901420083No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA9390.13749820622680908No Hit
GGACTGAACAGAGAGGACTTACCATGGAGTTTGGGCTGAGCTGGCTTTTT9270.13574104065202558No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAACTATGCCTTGAGTTGGG9110.1333981532189809No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT8990.1316409876441974No Hit
GTGTGAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGG8990.1316409876441974No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG8840.129444530675718No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA8770.12841951742376098No Hit
ACTATGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGATAATTCC8600.125930199526151No Hit
GATATCAAGAGCCCATGGCAGCCGGTTGGCCGCACAGTAGTATACGGCCG8580.12563733859702042No Hit
ATACTACTGTGCGGCCAACCGGCTGCCATGGGCTCTTGATATCTGGGGCC8500.1244658948804981No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT8130.1190479676915823No Hit
TTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGTTGGAGTCTGGGGGAGGCT8110.11875510676245171No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTAGCAACTATGCC7950.11641221932940705No Hit
GTTATAAATAAGTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGA7600.11128715306962184No Hit
CAGTAGTATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCAACTGCAGATA7550.11055500074679536No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC7400.108358543778316No Hit
CTTGATATCTGGGGCCAAGGGACAATGGTCACCGTCTCTTCAGCCTCCAC7340.10747996099092424No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT7280.10660137820353249No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG7180.10513707355787956No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT7160.10484421262874899No Hit
CACTTATTTATAACGGTGCTAGTAGCACCTACTATGCAGACTCCGTGAAG7120.10425849077048781No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC7070.10352633844766135No Hit
GTGCTAGTAGCACCTACTATGCAGACTCCGTGAAGGGCCGGTTCACCATC7070.10352633844766135No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG6980.10220846426657373No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG6930.10147631194374726No Hit
GAACAGAGAGGACTTACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT6920.10132988147918198No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC6890.1008905900854861No Hit
GATCAGGACTGAACAGAGAGGACTTACCATGGAGTTTGGGCTGAGCTGGC6870.10059772915635551No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC11250.051.232474
ATCAACG11300.050.3682023
CAACGCA11650.049.4734155
TATCAAC11800.048.2374952
AACGCAG12400.047.0310636
CGCAGAG12750.045.7400138
GAATACG3500.043.195749
GTATCAA14150.042.2661971
GGTATCA6100.037.7999881
GTAGTAT17450.037.163671
GCAGAGT15750.037.0249149
CTAGAGA4300.036.860574
AGTATAC16950.036.1290933
TAGTATA17650.035.5151982
TATACGG17600.034.794785
GCTAGAG4350.034.7806633
ATACGGC17900.034.1890756
CAGCGCT4850.034.140969
GTATACG17950.034.116334
TACGGCC18200.033.625527
Produced by FastQC (version 0.11.5)