FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280781

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280781
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences743961
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTCGGACCGGAGTCCACTGATGACCAGGGAGG29020.3900742108793337No Hit
GATTATTACTGTGCATCATGGGATGACAGCCTGAGTGCCCGCTGGGTGTT17930.24100725710084264No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC14810.19906957488362967No Hit
GTAATACACGGCCGTGTCCTCGGATCTCAGGCTGTTCATCTCCATGTAGG12680.17043904183149386No Hit
GTCCTGGGCCCAGTCTGTGTTGACTCAGCCACCCTCAGCGTCTGGGACCC11840.15914812738839804No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11770.1582072178514734No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGTTGACTCAGCCACCCTCAGCGTCT11730.15766955525894502No Hit
TATGTATACTGGTACCAACACCTCCCAGGAACGGCCCCCAAACTCCTCAT10440.14032993664990503No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGTTGACTCAGCCAC9350.1256786310035069No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT9140.12285590239273295No Hit
CAGTAATAATCAGCCTCATCCTCGGACCGGAGTCCACTGATGACCAGGGA9020.12124291461514784No Hit
TTATTACTGTGCATCATGGGATGACAGCCTGAGTGCCCGCTGGGTGTTCG8780.11801693905997761No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC8700.11694161387492087No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT8580.11532862609733574No Hit
GTGTTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC8400.11290914443095806No Hit
GACCTAGGACGGTCAGCTTGGTCCCGCCGCCGAACACCCAGCGGGCACTC8160.10968316887578783No Hit
GTCTGGGCCTGAGGTGAAGAAGCCTGGGACCTCAGTGAAGGTCTCCTGCA7890.10605394637622134No Hit
GTTCCTCACTATGGACTGGATTTGGAGGATCCTCTTCTTGGTGGGAGCAG7640.10269355517291902No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT7450.10013965785840924No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)