FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280782

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280782
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences743961
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTCGGACCGGAGTCCACTGATGACCAGGGAGG30060.4040534382850714No Hit
GATTATTACTGTGCATCATGGGATGACAGCCTGAGTGCCCGCTGGGTGTT17320.232807902564785No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC15200.20431178516078125No Hit
GTAATACACGGCCGTGTCCTCGGATCTCAGGCTGTTCATCTCCATGTAGG12450.16734748192445573No Hit
GACCTAGGACGGTCAGCTTGGTCCCGCCGCCGAACACCCAGCGGGCACTC11810.15874488044400176No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCGCCGCCGAACACCCA11110.14933578507475526No Hit
GTCCTGGGCCCAGTCTGTGTTGACTCAGCCACCCTCAGCGTCTGGGACCC10520.1414052618349618No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGTTGACTCAGCCACCCTCAGCGTCT10260.13791045498352736No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC9510.1278292813736204No Hit
GTATACATAATTACTTCCGATGTTGGAGCTGCTGCCAGAACAAGAGATGG9340.1255442153553748No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT9280.12473772146658224No Hit
CAGTAATAATCAGCCTCATCCTCGGACCGGAGTCCACTGATGACCAGGGA8970.12057083637448736No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT8760.11774810776371342No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA8590.11546304174546784No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT8580.11532862609733574No Hit
TATGTATACTGGTACCAACACCTCCCAGGAACGGCCCCCAAACTCCTCAT8550.11492537915293946No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGTTGACTCAGCCAC8540.11479096350480737No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC7860.10565069943182506No Hit
GTGTTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC7580.10188706128412646No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA7580.10188706128412646No Hit
TTATTACTGTGCATCATGGGATGACAGCCTGAGTGCCCGCTGGGTGTTCG7510.10094615174720181No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9200.053.2646831
ATCAACG8950.051.5213853
TCAACGC9000.050.43464
GTACGGA450.00990739247.9974256
CAACGCA9800.046.311265
CGCAGAG10350.043.823738
AACGCAG10500.043.197686
TATCAAC10800.042.0316622
GGTATCA5050.034.248231
GTACATG17650.033.8884471
GCAGAGT14800.031.1334639
ACATGGG17850.031.0801493
GTACACG4450.030.758545
ACGCAGA14750.030.7508897
TACGGAT1204.2262394E-429.998397
ACACGGG5000.028.798457
CGTAGAC1100.00996832626.1997953
ACGGGGT5550.025.9445519
CCTAGTA1751.3089713E-424.7043252
GTATGTA8850.024.428471
Produced by FastQC (version 0.11.5)