FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280784

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280784
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences812394
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC31600.38897382304645284No Hit
CTATTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGAC24670.303670386536582No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGCGGATGATCACTA23770.2925920181586767No Hit
CCTATGTGCTGCCTCAGCCACCCTCAGTGTCAGTGGCCCCAGGAAAGACG23470.28889922869937495No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGCGG21510.2647730042319367No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC20280.24963256744879947No Hit
GGATAATAGTGATCATCCGCTATTCGGCGGAGGGACCAAGCTGACCGTCC18840.23190717804415098No Hit
GTACATGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCA18200.22402922719764054No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT17800.21910550791857153No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC17000.20925806936043348No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA16810.20691930270287567No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT16770.2064269307749688No Hit
GTAATATACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA15000.18463947296508837No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA14990.18451637998311163No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAAATTTGGGAACACGG14700.1809466835057866No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGCCTCAGCCAC14530.17885410281218225No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA14010.17245326774939254No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG14000.1723301747674158No Hit
GGCTCTGTGACCTCCTATGTGCTGCCTCAGCCACCCTCAGTGTCAGTGGC13970.17196089582148563No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG13450.1655600607586959No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT13420.16519078181276572No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA13220.1627289221732312No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA13100.1612518063895105No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13060.1607594344616036No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC12550.15448169238079063No Hit
GCACAGGCTCTGTGACCTCCTATGTGCTGCCTCAGCCACCCTCAGTGTCA12390.152512204669163No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT12040.1482039502999776No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGCGGATGATCACTAT12030.14808085731800089No Hit
GGATTACCTGTGGGGGAAACAACATTGGAAGTAAAAGTGTGCACTGGTAC11880.14623446258835No Hit
GGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGGTGGTCATCGATTATGAT11740.14451116084067583No Hit
CCCCTGTGGTGGTCATCGATTATGATAGCGACCGGCCCTCAGGGATCCCT11680.14377260294881547No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11520.14180311523718786No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC11210.13798723279590938No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT11060.13614083806625848No Hit
GTATATTACTGTGCGCGAGCGCACCTCGGTGGCATTTTTTCCTGGGGCCA10880.13392516439067742No Hit
GTATCAACGCAGAGTACATGGGAGTCTGGGCCTAAGGAAGCAGCACTGGT10730.13207876966102655No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA10320.1270319573999808No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG10000.12309298197672558No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG9740.11989256444533071No Hit
GTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGG9730.11976947146335398No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG9530.11730761182381948No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA9390.11558431007614531No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC9350.11509193814823841No Hit
CCCTCAGTGTCAGTGGCCCCAGGAAAGACGGCCAGGATTACCTGTGGGGG9330.11484575218428496No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA9310.11459956622033152No Hit
GCCATGGCCTGGACCTTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGG9250.11386100832847115No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAG9200.11324554341858753No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG9190.1131224504366108No Hit
GGCCAGGCCCCTGTGGTGGTCATCGATTATGATAGCGACCGGCCCTCAGG8720.1073370802837047No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC8510.10475212766219347No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG8500.10462903468021674No Hit
CTATTACTGTCAGGTGTGGGATAATAGTGATCATCCGCTATTCGGCGGAG8400.10339810486044948No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT8140.10019768732905461No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13300.070.371511
TCAACGC13400.066.622684
ATCAACG13850.064.4580463
TATCAAC14950.060.1968842
CAACGCA15000.059.5162545
AACGCAG15800.056.5027776
GGTATCA3900.055.3811341
CGCAGAG16550.053.9422348
GCAGAGT20000.044.997179
TAACGAG700.001093450441.140275
ACGCAGA22350.039.9437987
TACATGG25300.038.9856952
GTACATG25600.038.5288281
CATGGGA13650.035.338444
ACATGGG27900.034.3204233
ACCGACC3050.033.0471048
TATTGAG13100.032.425445
ATGGGAG13350.031.2789335
GAATACG3350.030.0876629
TCTACAC8450.028.9685943
Produced by FastQC (version 0.11.5)