FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280785

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280785
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1469374
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAACAATTTCCCTTTCACTTTCGGCCCTGGGACCAAACTAGATATCA51600.351169954007625No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT48300.3287114104373699No Hit
CTCTTATAGTGGGAGCACTTACTACAACCCGTCCCTCAAGAGTCGAGTCA46680.3176863072301538No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39870.2713400400442637No Hit
TTGTTATACTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCT35700.24296060771457775No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAAGTTCAGCG31630.2152617373112632No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGACATTTACAA28980.19722684626242193No Hit
CTTATAGTGGGAGCACTTACTACAACCCGTCCCTCAAGAGTCGAGTCACC28220.19205457562199957No Hit
GTGTCTGGTGGCTCCATCAGCAGTAGTAGTTTCTACTGGGGCTGGATCCG27330.1859975744772944No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26240.17857944947984652No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23800.1619737384763852No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG23350.15891120980771403No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22460.15285420866300886No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21890.14897500568269206No Hit
CAGTATAACAATTTCCCTTTCACTTTCGGCCCTGGGACCAAACTAGATAT20920.14237355499688983No Hit
CACCTGCACTGTGTCTGGTGGCTCCATCAGCAGTAGTAGTTTCTACTGGG20250.13781379009020167No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT20230.1376776777049274No Hit
GTAAAACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA20080.13665683481537036No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18450.12556367541551708No Hit
GTGTTTTACTGTGCGAGATTACTAGCATTGGGTTACGATTTTTGGAGTGG18330.12474700110387145No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT18300.12454283252596003No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG18250.12420255156277435No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT18220.12399838298486293No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGG18180.12372615821431439No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG17940.12209280959102312No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17750.12079974193091753No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA17740.12073168573828039No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC17630.11998306761927188No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17140.11664831418005218No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG16720.11378995408929246No Hit
GTCCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAAGT16580.11283716739237254No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG16500.11229271785127544No Hit
CTATAAGAGATACTCCCAATCCACTCCAGCCCCCTCCCTGGGGGCTGGCG16440.11188438069545262No Hit
GTCTCCATCCTCACTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT16430.11181632450281548No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA16390.11154409973226695No Hit
GTTATACTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGC16330.11113576257644413No Hit
TATTTAGCCTGGTTTCAGCAGAAACCAGGGAAAGCCCCTAAGTCCCTGAT15970.10868573964150721No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC15780.10739267198140161No Hit
AGTCAGGACATTTACAATTATTTAGCCTGGTTTCAGCAGAAACCAGGGAA15450.1051468176243761No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15250.10378569377163335No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA14860.10113150225878502No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11450.072.467611
GTATCAA33900.067.682911
TCAACGC35800.062.776594
ATCAACG36350.061.8267443
TATCAAC37850.059.186232
CAACGCA38100.059.1699455
AACGCAG39800.056.781016
ACGCAGA43050.052.4926227
CGCAGAG44100.051.2427988
GCAGAGT50850.044.5821889
GAATACG4550.039.5430689
GTGTAGA6750.035.274571
GTGCTAG6600.031.7033841
GTACATG38000.031.3293911
TACATGG38450.030.1616952
CAGAGTA51150.027.0145269
GCTAGAG7600.025.5903573
CTAGAGA7900.025.530374
CTATAGA5250.023.3246864
ACATTAG13300.023.2679757
Produced by FastQC (version 0.11.5)