FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280786

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280786
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1469374
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAACAATTTCCCTTTCACTTTCGGCCCTGGGACCAAACTAGATATCA51740.35212274070454497No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT44440.3024417200794352No Hit
CTCTTATAGTGGGAGCACTTACTACAACCCGTCCCTCAAGAGTCGAGTCA42160.28692490815816807No Hit
TTGTTATACTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCT35930.2445259001452319No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32280.21968538983267705No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAAGTTCAGCG29530.20096993685746445No Hit
GTGTCTGGTGGCTCCATCAGCAGTAGTAGTTTCTACTGGGGCTGGATCCG26070.17742249420501519No Hit
CTTATAGTGGGAGCACTTACTACAACCCGTCCCTCAAGAGTCGAGTCACC24070.16381125567758786No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGACATTTACAA23770.16176956989847377No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23330.15877509742243975No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20790.14148882449260705No Hit
CAGTATAACAATTTCCCTTTCACTTTCGGCCCTGGGACCAAACTAGATAT20700.14087631875887283No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20420.138970745365033No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG19410.1320970699086822No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT19170.1304637212853909No Hit
GTGTTTTACTGTGCGAGATTACTAGCATTGGGTTACGATTTTTGGAGTGG18690.12719702403880837No Hit
CACCTGCACTGTGTCTGGTGGCTCCATCAGCAGTAGTAGTTTCTACTGGG18280.12440672014068575No Hit
GTAAAACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA18190.12379421440695153No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18020.12263725913212022No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT17490.11903028092235196No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG17330.11794138184015779No Hit
GTTATACTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGC17230.11726081991378641No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT16720.11378995408929246No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16520.11242883023654972No Hit
GTCCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAAGT16400.11161215592490409No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGG16270.1107274254206213No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG15680.10671211005503023No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA15520.10562321097283606No Hit
GTCTCCATCCTCACTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT15380.10467042427591614No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA15250.10378569377163335No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA15170.10324124423053627No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA14930.10160789560724498No Hit
CTATAAGAGATACTCCCAATCCACTCCAGCCCCCTCCCTGGGGGCTGGCG14890.10133567083669645No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14810.10079122129559935No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)