FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280790

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280790
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1833882
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC32490.17716516111723654No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA21670.11816463654695339No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT19360.1055684062551462No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG35750.046.734583
GTATCAA39100.045.686121
TCAACGC36850.045.338284
TATCAAC44450.039.049822
CGCAGAG42450.039.0062648
GCCCATA9950.035.471721
CAACGCA49750.033.5822225
GCAGAGT49950.033.1494679
AATCCGC9350.033.1095246
AACGCAG50750.032.9115226
GTACACG17850.031.8715655
TACATGG61650.031.3094522
GTACATG63700.030.9827861
GCGTAAT950.004881358230.3281361
AAATCCG9850.029.9752035
ACATGGG62500.029.7280253
CATGGGG35050.029.5861874
TACACGG19350.029.3928816
ACGCAGA60100.027.9111337
GTATGTA27200.025.15731
Produced by FastQC (version 0.11.5)