FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280792

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280792
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1018735
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATTACTTATCCCTTCACTTTCGGCGGAGGGACCGGGGTGGAGATCA69590.6831020824846501No Hit
GTAATATACTGCTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCT56890.5584376702479055No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT40520.3977481877033773No Hit
ACATATTACTGTGCGAGAGAAGGTGAGGGATTTTGTAGTAGTGTCACCTG38200.374974846255405No Hit
GTAATATGTAGCCGTGTCCTCGTCTCTCAGACTATTCATTTGCAGGAACA37400.3671219698940352No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31200.30626217809341977No Hit
GTATGTAGCCTGGTTTCAGCAGAAACCAGGGAAAGCCCCTAAGTCCCTGA21640.21242030557505143No Hit
GATCTATGCTGCATCTAGTTTGCAAAGTGGGGTCCCTTCAAAGTTCAGCG20610.20230972725978788No Hit
CACCATAAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGCCAGC20470.20093547389654817No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGACATTAGAAT20230.19857961098813723No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19160.18807638885480524No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18470.18130328299312384No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT16580.1627508625893878No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG16190.15892258536322007No Hit
ATATTACTTATCCCTTCACTTTCGGCGGAGGGACCGGGGTGGAGATCAAA15930.1563704005457749No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15930.1563704005457749No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC15640.15352373286477838No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15470.15185499663798732No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGG14970.14694694891213123No Hit
CATTTCACTCTCACCATAAGCAGCCTGCAGCCTGAAGATTTTGCAACTTA14520.14252970595886072No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13740.13487315150652526No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13460.13212464478004585No Hit
CTCCAGAGACAATGCCAAGAATTCACTGTTCCTGCAAATGAATAGTCTGA13340.13094671332584037No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13150.12908165519001508No Hit
GTCTCCATCCTCACTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT13150.12908165519001508No Hit
GCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGT13060.128198206599361No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13010.12770740182677537No Hit
CATTAGTAGTAGTAGTGCTACCATTTACTACGCAGACTCTGTGAAGGGCC12990.12751107991774113No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA12540.12309383696447063No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA12510.12279935410091929No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12460.12230854932833368No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12360.12132693978316245No Hit
GGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCC12320.12093429596509396No Hit
AGTCAGGACATTAGAATGTATGTAGCCTGGTTTCAGCAGAAACCAGGGAA12270.12044349119250836No Hit
GTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGC12220.11995268641992274No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG11650.1143575120124468No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11620.11406302914889543No Hit
GTCCCTGATCTATGCTGCATCTAGTTTGCAAAGTGGGGTCCCTTCAAAGT11590.11376854628534408No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC11520.11308141960372421No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11350.11141268337693316No Hit
AAGTAATATACTGCTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGG11340.11131452242241603No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11250.11043107383176193No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT11060.10856601569593663No Hit
ACTTATCCCTTCACTTTCGGCGGAGGGACCGGGGTGGAGATCAAACGAAC11050.10846785474141951No Hit
ACCATTTACTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGA10950.10748624519624829No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG10910.10709360137817979No Hit
GGACATTAGAATGTATGTAGCCTGGTTTCAGCAGAAACCAGGGAAAGCCC10770.10571934801494011No Hit
GTACATGGGAGCTCTCAGAGAGGTGCCTTAGCCCTGGATTCCAAGGCATT10670.10473773846976887No Hit
ATGCTGCATCTAGTTTGCAAAGTGGGGTCCCTTCAAAGTTCAGCGGCAGT10670.10473773846976887No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG10640.1044432556062175No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10640.1044432556062175No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA10600.10405061178814902No Hit
CTCCTGTGCAGCCTCTGAATTCACCTTCAGTTCCTATGGCATGAATTGGG10450.10257819747039221No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA10320.10130210506166962No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC10280.10090946124360113No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14950.060.650851
TCAACGC16300.054.7015274
ATCAACG16550.054.2909933
CAACGCA17500.051.3639875
TATCAAC18300.048.706442
AACGCAG18400.048.4703526
GGTATCA8300.045.085071
CGCAGAG20100.044.370878
GCAGAGT22500.039.9576349
GTACATG28100.036.10941
TACATGG28500.034.805672
TTACTTA35300.031.9887456
GTATATT36500.031.7424581
TACTTAT35600.031.7191777
ACTTATC35900.031.4541178
TATATTA37900.030.5353182
CTTATCC37100.030.4367339
GTAATAT44150.030.317251
GAGTTTA4100.029.8381981
ATTACTT38300.029.6650495
Produced by FastQC (version 0.11.5)