FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280793

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280793
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1547335
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC24630.15917690739238755No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24360.15743197174496795No Hit
GTAATATACGGCCGTGTCCTCGGCACTCAGGCTGTTCATTTGCAGAGACA23730.15336045523432223No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA20200.13054703732546605No Hit
GTCATAGGACTGACAGTAATAATCAGCCTCATCTTCAGCCTGGAGCCCAG18000.11632904316130638No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17640.11400246229808025No Hit
GCATAATATGTGCTACTACCACCGTTTAACATAACTGAGACCCACTCCAG15900.10275732145915396No Hit
GTATATTACTGCGCGAGGCTCCGACCGTTTGGTGGGAGCTACTACATTGA15830.1023049307357489No Hit
ATATACGGCCGTGTCCTCGGCACTCAGGCTGTTCATTTGCAGAGACAGCG15660.10120626755033654No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG39900.056.8438153
TCAACGC40850.055.5182764
TATCAAC43250.052.773842
GTATCAA44950.052.239851
CAACGCA43550.052.076275
AACGCAG46250.048.880476
CGCAGAG46900.048.508488
GCAGAGT56350.040.6290479
ACGCAGA58150.039.123787
GGTATCA23600.038.151541
GTACATG46400.034.6179121
ACATGGG47000.032.171183
TACATGG47750.032.1182442
CCGATCG900.00375514431.997869
CATGGGA27950.028.8505254
GTACACG13300.028.6907395
TACACGG13350.028.5832826
ACACGGG14200.026.871447
CAGAGTA56100.026.0517359
CATGGGG20000.024.8391424
Produced by FastQC (version 0.11.5)