FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280795

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280795
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences950579
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT34800.36609266562800147No Hit
GTATGGAGACTCTCCATTCACTTTCGGCCCTGGGACCACAGTGGATATCA31840.33495374924125193No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26840.28235422831768847No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC21730.22859751793380664No Hit
GTCTCCAGGCATTCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT18230.19177785328731226No Hit
GTAATATACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA18050.18988427053406398No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17340.18241513856291797No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16880.17757598263795013No Hit
GTATATTACTGTGCGACCGACTATGGTTCGGGGAGTTATTATAACGACTG15650.1646365004907535No Hit
GTCTGGGACAGACTTCACTCTCACCATCAACAGACTGGAGCCTGACGATT14940.15716736851960753No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG14770.15537898480820636No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAACAACCACTTAGCCTGGTACC14470.15222301355279255No Hit
GTCTCCATACTGCTGACAGAAATACACAGCAAAATCGTCAGGCTCCAGTC14410.15159181930170978No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14300.1504346298413914No Hit
TCTCTGGTGCATCCAGCTGGGCCACTGGCGTCCCAGACAGGTTCAGAGGC14030.14759425571151896No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13410.14107191511699713No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT12720.13381318122954536No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12240.12876362722088328No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12160.12792203488610626No Hit
CTCCAGGCATTCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC11780.12392447129591544No Hit
GTCCCAGACCCACTGCCTCTGAACCTGTCTGGGACGCCAGTGGCCCAGCT11540.12139969429158438No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA11370.11961131058018323No Hit
TCTCCATACTGCTGACAGAAATACACAGCAAAATCGTCAGGCTCCAGTCT11360.11950611153833611No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA11130.1170865335758522No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10700.11256297477642574No Hit
GTCAGAGTGTTAGCAACAACCACTTAGCCTGGTACCAGCAGAAACCTGGC10130.1065666293911395No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9960.10477824567973834No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA9900.10414705142865559No Hit
GTTCAGAGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAACAGAC9840.10351585717757283No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9760.10267426484279582No Hit
GACTTCACTCTCACCATCAACAGACTGGAGCCTGACGATTTTGCTGTGTA9520.10014948783846477No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)