FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280797

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280797
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1085981
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATATACTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGA74170.6829769581604097No Hit
GTAATACACAGCCGTGTCCTCGTCTCTCAGGCTGTTCATTTGCAGATACA65630.6043383816107281No Hit
GTATATGGTACTACTACTACTACTAATGTATGAAACCCACTCCAGCCCCT56520.5204510944482454No Hit
GTGTATTACTGTGCGAGAGATGGACCCGCGTTTTTGGAGTGGTTACCTCA40380.3718297097278866No Hit
TAGTAGTACCATATACTACGCAGACTCTGTGAAGGGCCGATTCACCATCT27880.25672640681558884No Hit
GTACTACTACTACTACTAATGTATGAAACCCACTCCAGCCCCTTCCCTGG26590.24484774595503975No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT24090.2218270853725802No Hit
ATACTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAATG23950.22053792837996244No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT23630.21759128382540763No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA23230.2139079781322141No Hit
GTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGC21250.19567561495090613No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC21170.19493895381226745No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA19510.1796532351855143No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC19370.17836407819289657No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG18960.1745886898573732No Hit
GCGTAGTATATGGTACTACTACTACTACTAATGTATGAAACCCACTCCAG17740.16335460749313294No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG17650.1625258637121644No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC17180.158197979522662No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA16570.15258093834054187No Hit
CAGTAATACACAGCCGTGTCCTCGTCTCTCAGGCTGTTCATTTGCAGATA16490.15184427720190316No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT16210.14926596321666769No Hit
ATACACAGCCGTGTCCTCGTCTCTCAGGCTGTTCATTTGCAGATACAGTG15820.145674740165804No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT15780.14530640959648466No Hit
GTATTACTGTGCGAGAGATGGACCCGCGTTTTTGGAGTGGTTACCTCAAC15630.14392516996153706No Hit
CTCCAGAGACAATGCCAAGAACTCACTGTATCTGCAAATGAACAGCCTGA15430.1420835171149403No Hit
CATTAGTAGTAGTAGTAGTACCATATACTACGCAGACTCTGTGAAGGGCC14980.13793979821009758No Hit
GAGTTGGGGCTGTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCA14410.1326910875972968No Hit
GTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGG14310.13177026117399845No Hit
TTTAGAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCT13880.1278107075538154No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGCTATAGCATGAACTGGG13410.12348282336431302No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13340.12283824486800414No Hit
GTACATGGGAGCTCTCAGAGAGGTGCCTTAGCCCTGGATTCCAAGGCATT13270.12219366637169528No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT13240.12191741844470576No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGCTATAGC13070.12035201352509851No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA12780.1176816168975332No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG12690.11685287311656466No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT12600.11602412933559611No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG12510.11519538555462756No Hit
GAACACAGAGGACTCACCATGGAGTTGGGGCTGTGCTGGGTTTTCCTTGT12500.11510330291229773No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGGCTGTGCTGGGTTTTC12360.11381414591968No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC12240.11270915421172194No Hit
ATATGGTACTACTACTACTACTAATGTATGAAACCCACTCCAGCCCCTTC11990.11040708815347598No Hit
CTACTAATGTATGAAACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGAC11970.1102229228688163No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA11890.10948626173017759No Hit
GGGTGCCACGGCCCCAGAGATCGAAGTACCACGGTTGAGGTAACCACTCC11780.10847335266454938No Hit
GCCCCAGAGATCGAAGTACCACGGTTGAGGTAACCACTCCAAAAACGCGG11680.107552526241251No Hit
GCTGTGTATTACTGTGCGAGAGATGGACCCGCGTTTTTGGAGTGGTTACC11590.10672378246028245No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT11560.10644753453329295No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC11340.10442171640203651No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG11240.10350088997873812No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC11130.1024879809131099No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG11090.10211965034379056No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG11070.10193548505913086No Hit
GTACCATATACTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGA10940.10073841070884297No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA10900.10037008013952362No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15400.063.612141
ATCAACG15950.060.0333753
TCAACGC16150.059.2899324
CAACGCA17250.055.9264875
AACGCAG18550.052.0071076
TATCAAC19300.049.6130752
CGCAGAG21500.045.2061128
GAATACG2150.043.5318039
CAGCGCT6750.039.463889
GCAGAGT25500.038.1149529
GGCAGCG6800.035.9974567
GTACATG22700.035.8570371
TACATGG22500.035.517492
ACATGGG23200.034.445843
TATACTA34750.034.3918155
ACTACGC34450.034.0643548
CTACGCA34900.033.831429
GCAGCGC7450.033.8231128
TACTACG34850.033.6733747
ATATACT36800.033.2585224
Produced by FastQC (version 0.11.5)