FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280798

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280798
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1085981
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATATACTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGA72330.6660337519717197No Hit
GTAATACACAGCCGTGTCCTCGTCTCTCAGGCTGTTCATTTGCAGATACA66200.6095870922235288No Hit
GTATATGGTACTACTACTACTACTAATGTATGAAACCCACTCCAGCCCCT59570.548536300358846No Hit
GTGTATTACTGTGCGAGAGATGGACCCGCGTTTTTGGAGTGGTTACCTCA40130.36952764366964064No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT27670.2547926713266623No Hit
TAGTAGTACCATATACTACGCAGACTCTGTGAAGGGCCGATTCACCATCT27120.24972812599852115No Hit
GTACTACTACTACTACTAATGTATGAAACCCACTCCAGCCCCTTCCCTGG25440.23425824208710833No Hit
ATACTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAATG24470.22532622578111403No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA21920.20184515198700528No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT21810.20083224292137708No Hit
GTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGC21720.20000349914040855No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA20140.1854544416522941No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC20050.18462569787132554No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC18640.17164204530281837No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG18010.16584083883603856No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG17270.15902672330363055No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC17270.15902672330363055No Hit
GCGTAGTATATGGTACTACTACTACTACTAATGTATGAAACCCACTCCAG16790.1546067564717983No Hit
CAGTAATACACAGCCGTGTCCTCGTCTCTCAGGCTGTTCATTTGCAGATA16500.151936359844233No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA16450.1514759466325838No Hit
CTCCAGAGACAATGCCAAGAACTCACTGTATCTGCAAATGAACAGCCTGA16160.14880555000501852No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT15840.14585890545046368No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT15760.145122244311825No Hit
ATACACAGCCGTGTCCTCGTCTCTCAGGCTGTTCATTTGCAGATACAGTG15340.1412547733339718No Hit
GTATTACTGTGCGAGAGATGGACCCGCGTTTTTGGAGTGGTTACCTCAAC14940.13757146764077827No Hit
GAGTTGGGGCTGTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCA14640.1348089883708831No Hit
CATTAGTAGTAGTAGTAGTACCATATACTACGCAGACTCTGTGAAGGGCC14520.13370399666292504No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT14290.13158609588933878No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT14230.13103360003535974No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA14220.1309415173930299No Hit
GTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGG13710.12624530263420813No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13510.12440364978761138No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGCTATAGCATGAACTGGG13500.12431156714528153No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG13110.12072034409441786No Hit
GTACATGGGAGCTCTCAGAGAGGTGCCTTAGCCCTGGATTCCAAGGCATT12930.11906285653248078No Hit
TTTAGAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCT12930.11906285653248078No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT12440.1145508070583187No Hit
CTACTAATGTATGAAACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGAC12270.11298540213871146No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGCTATAGC12240.11270915421172194No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC12100.1114199972191042No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG11950.11003875758415663No Hit
ATATGGTACTACTACTACTACTAATGTATGAAACCCACTCCAGCCCCTTC11880.10939417908784776No Hit
GAACACAGAGGACTCACCATGGAGTTGGGGCTGTGCTGGGTTTTCCTTGT11880.10939417908784776No Hit
GCCCCAGAGATCGAAGTACCACGGTTGAGGTAACCACTCCAAAAACGCGG11790.10856543530687922No Hit
GGGTGCCACGGCCCCAGAGATCGAAGTACCACGGTTGAGGTAACCACTCC11760.10828918737988971No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC11740.10810502209523004No Hit
GTACCATATACTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGA11470.10561879075232439No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT11300.10405338583271714No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG11180.10294839412475908No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG11160.1027642288400994No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT11160.1027642288400994No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGGCTGTGCTGGGTTTTC10990.10119882392049216No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA10960.10092257599350264No Hit
GTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTG10870.1000938322125341No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC10300.062.231014
ATCAACG11050.058.0071833
GTATCAA12350.056.5820771
CAACGCA11800.054.3202865
TATCAAC12500.052.4379232
AACGCAG12950.050.5947726
CGCAGAG15400.041.1430058
GGCAGCG5350.033.6449787
GCAGAGT19200.033.3751149
CAGCCGT30750.033.014759
GTACATG22350.032.877351
TACATGG21700.032.8616642
TATACTA36700.032.7721145
TACACGG11550.032.4156956
GTACACG11600.032.2848975
GCAGCGC6250.032.2561158
CTACGCA35050.031.634929
ACTACGC35050.031.634928
TACTACG35700.031.2606147
GTAATAC33450.031.228131
Produced by FastQC (version 0.11.5)