FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280800

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280800
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences779939
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC17570.22527402784063882No Hit
GCTTGATACCACTGCTTGATACCACTGCTTGATACCACTGCTTGATACCA17000.21796576398923506No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG15370.19706669367732604No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT15070.19322023901869248No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAAAAAAGTAG14900.19104058137880015No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT14890.19091236622351235No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA14760.18924556920477115No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAAAAAAGTAGT14730.18886092373890778No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA14470.18552732970142535No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC14260.18283481144038188No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTC13970.1791165719370361No Hit
GTATCAAGCAGTGGTATCAAGCAGTGGTATCAAGCAGTGGTATCAAGCAG13960.1789883567817483No Hit
GATTATTACTGCAGCTCATATGCAGGCAGCAACTACTTTTTTTATGTCTT13500.1730904596385102No Hit
GTCAATAAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTC12890.1652693351659553No Hit
GCTTATAACTATGTCTCCTGGTACCAACACCACCCAGGCAAAGCCCCCAA12030.15424283181120574No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT11740.15052459230785997No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA11740.15052459230785997No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCAGCTCATAT10540.13513877367332575No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10510.13475412820746238No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC10390.13321554634400895No Hit
CTTATTGACCGCATAAATGATGAGTTTGGGGGCTTTGCCTGGGTGGTGTT10210.13090767354882882No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC10200.13077945839354105No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA9880.12667657342433192No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT9850.12629192795846855No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTG9680.12411227031857619No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC9640.12359940969742506No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA9620.12334297938684949No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGCTTATAACTATGTCTCCT9560.12257368845512277No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT9410.12065046112580599No Hit
GTTATAAGCACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGACTG9320.11949652472821592No Hit
GTACCAACACCACCCAGGCAAAGCCCCCAAACTCATCATTTATGCGGTCA9080.11641936100130908No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC8700.11154718510037323No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG8420.10795716075231525No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT8250.1057775031124229No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA7910.1014181878326382No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATA7820.10026425143504812No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACCG855.5598368E-542.3569565
GAATACG2302.910383E-1134.4314279
TATAGCG850.002834905633.8855675
GACCGCG1306.7531277E-427.6896087
AATCCGC1400.001041026725.7117796
TCCGCTA1701.08136344E-425.4092868
GTGCTAG3551.6916601E-1024.3418981
ATCCGCT1851.9193077E-423.3490757
GTCTCGC2802.4990732E-723.1465381
CAATCCG2202.3522001E-522.9068579
CCGCTAC1902.2992677E-422.7346239
GTTCTAA3203.634341E-822.5035781
AAATCCG1600.002262713422.5021345
TATTGGG7050.022.4716572
GTATAAC2252.7926857E-522.4035631
ATACACT5750.021.2889754
CGCTCCG1700.003217044521.1784785
TATACTG6300.020.5733815
ATAGGAC4201.378794E-920.573383
ATTGGGG6700.020.4198473
Produced by FastQC (version 0.11.5)