FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280801

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280801
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1019346
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT28960.2841037292538549No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25200.24721733346675223No Hit
ACAGTACCCCTCGCACTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGA16670.1635362281305857No Hit
GAGTTACAGTACCCCTCGCACTTTTGGCCAGGGGACCAAGCTGGAGATCA16540.1622609006166699No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16400.16088747098629905No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16250.15941593923947314No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15450.1515677699230683No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15160.14872280854587158No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCGGTG15140.14852660431296144No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG15110.14823229796359627No Hit
GTAGGAGACAGGGTCACCATCACTTGCCGGGCAAGTCAGTACATTAGCAA13950.13685245245480926No Hit
GTAATAGGTGGCTGTGTCCACAGGGTCCATGTTGGTCATTGTAAGGACCA13010.12763085350803358No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12930.1268460365763931No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12490.12252954345237045No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA12130.11899786725998826No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11820.11595670164988141No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG11790.11566239530051621No Hit
GGGTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGC11630.11409276143723524No Hit
CTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTT11190.10977626831321259No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAA10850.10644079635374053No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10820.10614649000437536No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10390.10192809899680776No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC10310.10114328206516728No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT10210.10016226090061667No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACT10200.10006415878416161No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC22500.049.2847864
TCCGGTA450.00989870148.0093732
CAACGCA23400.047.6969385
ATCAACG23400.047.3938673
TATCAAC23800.047.202492
GTATCAA25450.045.5771561
AACGCAG24600.045.077556
GAATACG4100.043.900249
ACGCAGA26300.042.1575857
CGCAGAG27300.040.613358
GTGCTAG5300.036.702611
GCAGAGT32000.035.323239
GTACATG28300.034.368171
GTATACG850.002827787533.903941
TACATGG27900.033.0387082
GCTAGAG6550.029.6851843
CTAGAGA7100.029.4113084
ACATGGG31450.028.851423
GTTAGAC1000.006277391728.8056243
CATGGGG18100.026.654524
Produced by FastQC (version 0.11.5)