FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280802

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280802
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1019346
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT29780.29214810280316983No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24550.24084069589717327No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16670.1635362281305857No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA16410.1609855731027541No Hit
GAGTTACAGTACCCCTCGCACTTTTGGCCAGGGGACCAAGCTGGAGATCA16070.15765010114328207No Hit
ACAGTACCCCTCGCACTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGA16050.15745389691037195No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16040.15735579479391687No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15660.15362791436862458No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15250.14960572759396712No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCGGTG14710.14430821330539387No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG13640.1338112868447024No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13480.13224165298142143No Hit
GTAATAGGTGGCTGTGTCCACAGGGTCCATGTTGGTCATTGTAAGGACCA13460.1320454487485113No Hit
GTAGGAGACAGGGTCACCATCACTTGCCGGGCAAGTCAGTACATTAGCAA13150.12900428313840442No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT12280.12046939900681418No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG11900.11674151858152186No Hit
GGGTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGC11800.11576049741697128No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC11030.10820663444993163No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10930.10722561328538102No Hit
CTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTT10810.1060483878879203No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10720.10516546883982475No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10280.10084897571580209No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAA10260.10065277148289196No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10230.1003584651335268No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC13850.041.591554
ATCAACG14500.039.729053
TAAACCG750.001534147438.4028665
CAACGCA15550.037.0445635
GTGCTAG5050.035.6584741
AACGCAG16650.035.021056
TATCAAC16850.034.6206552
ACGCAGA16850.034.605377
GAATACG3700.033.075439
CGCAGAG17600.032.3126688
GTATCAA19650.031.1580681
TCTACAC11800.028.6815133
GTACATG24600.028.4021191
TTACACG1050.00797035627.4306184
TACGTGT2105.30541E-727.423897
TACATGG24550.027.282342
GCAGAGT23750.024.2484939
TGCTAGA8450.023.8644522
GCTAGAG6350.023.8139693
ACATGGG28300.023.4092563
Produced by FastQC (version 0.11.5)