FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280806

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280806
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences876982
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATTACTGTGCCCACATGACGACGTACTTCTTTGACTTCTGGGGCCA28270.3223555329527858No Hit
GTAATACACAGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGAAGAAACA26280.2996640752033679No Hit
GTATTACTGTGCCCACATGACGACGTACTTCTTTGACTTCTGGGGCCAGG19400.2212132062003553No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT19030.21699419144292584No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA17270.19692536448866682No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT16580.18905747210319024No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC15490.17662848268265482No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC15000.17104113881470773No Hit
AACACATACTACGCAAACTCCGTGAAGGGCAGATTCACCATCTCCAGGGA14760.1683044805936724No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA14750.1681904531677959No Hit
CAGTAATACACAGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGAAGAAA13170.15017411987931337No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC12240.13956956927280148No Hit
CTCCAGGGACAAGTCCAAGAACACGCTGTTTCTTCAAATGAACAGCCTGA12170.1387713772916662No Hit
GTCCAAGAACACGCTGTTTCTTCAAATGAACAGCCTGAGAGTCGAGGACA12160.13865734986578973No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA11860.13523652708949557No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG11740.1338681979789779No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA11680.1331840334237191No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT11380.12976321064742494No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11060.12611433301937783No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT11040.12588627816762488No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG10560.12041296172555423No Hit
GAACACAGAGGACTCGCCATGGAGTTTGGGCTGAGCTGGGTTTTCCTTGT10360.11813241320802478No Hit
GTGTAGCCTCTGGATTCAGCGTCGATAGCAACTACATGAGCTGGGTCCGC9910.11300117904358357No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT9900.1128871516177071No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC9840.11220298706244826No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT9780.11151882250718943No Hit
GTGTATAGCGGTGGTAACACATACTACGCAAACTCCGTGAAGGGCAGATT9570.10912424656378353No Hit
GATCTGCACTGAACACAGAGGACTCGCCATGGAGTTTGGGCTGAGCTGGG9430.10752786260151291No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC9320.10627356091687172No Hit
GTATAGCGGTGGTAACACATACTACGCAAACTCCGTGAAGGGCAGATTCA9250.10547536893573642No Hit
TTCTTGGACTTGTCCCTGGAGATGGTGAATCTGCCCTTCACGGAGTTTGC9090.10365093012171288No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10250.059.6710551
TCAACGC10200.058.5325624
CAACGCA10300.057.259375
ATCAACG10650.056.734773
TATCAAC10750.055.5378722
AACGCAG10650.055.3459636
GGTATCA4400.049.0610921
CGCAGAG13000.044.859958
GTACATG20450.035.890171
TACATGG20050.035.517342
GCAGAGT16600.034.6975679
ACATGGG20200.034.54143
ACGCAGA17250.033.80757
GGGCAGT6750.031.9805641
GTAATAC17300.030.363051
AATACAC18150.029.3274773
CAGCCGT17900.028.9598679
TAATACA19350.027.1369782
CATGGGA10550.025.9090064
CATGGGG14500.025.7961524
Produced by FastQC (version 0.11.5)