FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280810

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280810
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences870028
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACTACTGTGCAAGCCTGGATTACCACAACTACTACGGTATGGACGT29820.3427475897327443No Hit
GTAGTACACAGCCGTGTCCTCGGCTTTCAGACTGTTCATTTGTAGATACA25980.2986110791836585No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16840.1935569889704699No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15370.176660981025898No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT14840.17056922305948774No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13710.1575811353197828No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC13360.15355827628536092No Hit
GTATTACTGTCAGCAGTATGGTAGGTCACCGATCACCTTCGGCCAAGGGA13200.15171925501248237No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC13050.1499951725691587No Hit
GAGTAGCACAACCTACGCGGACTCCGTGAAGGGCCGATTCACCGTCTCCA12460.14321378162541895No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA11930.13712202365900866No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGG11430.13137508218126312No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC10970.12608789602173723No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG10430.11988119922577203No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT10310.11850193327111311No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10250.11781230029378364No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA10030.1152836460435756No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT9910.11390438008891668No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA9860.11332968594114212No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT9520.10942176573627514No Hit
TTTAAAAGGTGTCCAGTGTGAGGTGCAGTTGGTGGAGTCCGGGGGAGGCC9480.10896201041805552No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9360.10758274446339658No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG9350.10746780563384167No Hit
GTATTAATATTGATGGGAGTAGCACAACCTACGCGGACTCCGTGAAGGGC9050.10401964074719436No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT8990.10333000776986487No Hit
GCTGTGTACTACTGTGCAAGCCTGGATTACCACAACTACTACGGTATGGA8850.10172086415609612No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT8840.10160592532654121No Hit
ACCTACGCGGACTCCGTGAAGGGCCGATTCACCGTCTCCAGAGACAACGC8710.10011172054232738No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC11750.064.878194
ATCAACG11700.064.537043
GTATCAA12200.064.2832261
CAACGCA13150.057.9715
AACGCAG13550.057.8219346
TATCAAC14400.052.445422
GGTATCA5550.051.8558771
CGCAGAG15800.049.6822558
GCAGAGT18850.041.643489
ACGCAGA19150.041.367177
GAATACG1303.8967846E-738.7781079
TACATGG21550.031.707142
GTACATG22900.030.1624581
ACATGGG22500.029.723923
CAGCCGT16050.029.6142549
GTGTACG1000.00630421428.7800121
CCTATAC2253.0478986E-828.7650833
AGTACAC17050.028.6807293
TAGTACA18150.027.7397252
GTAGTAC17650.027.7201251
Produced by FastQC (version 0.11.5)