FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280811

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280811
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences863037
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGAAGA25740.2982490901317093No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT22630.2622135551546458No Hit
GTATATTACTGTGCGAGAGTAAACTGTATTAGTACCAGTTGCTGGGGGTA22520.26093898639339913No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17820.20648013932195258No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA16480.19095357441222102No Hit
GTATTAGTACCAGTTGCTGGGGGTACTTTGACTATTGGGGCCAGGGAACC15340.1777444072502106No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15220.1763539686015779No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC14620.16940177535841452No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13930.16140675312877664No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13750.15932109515582762No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC13130.15213716213789213No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA12440.14414213990825422No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC12300.14251996148484944No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT12150.14078191317405858No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAA12080.1399708239623562No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11800.1367264671155466No Hit
GCCGTATATTACTGTGCGAGAGTAAACTGTATTAGTACCAGTTGCTGGGG11510.13336624038135098No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11410.13220754150749042No Hit
ATATACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGAAGACTG10840.12560295792648518No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG10230.11853489479593574No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG10160.11772380558423336No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG10120.11726032603468914No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAGGGTTCAGTG10100.11702858625991701No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10050.11644923682298672No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10040.11633336693560067No Hit
GAGTTACAGTTCCCCTCGGACGTTCGGCCAGGGGACCAAGGTGGAAATCA9790.11343661975094926No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9650.11181444132754446No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9590.11111922200322814No Hit
CATAACAACCACATTCCTCCTCTAAAGAAGCCCCTGGGAGCACAGCTCAT9340.10822247481857672No Hit
GTCTTCATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTATATTA9250.10717964583210221No Hit
ATCTGGGACAGAGTTCACTCTCACCATCTCCAGTCTGCGACCTGAAGATT9170.10625268673301377No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT8860.10266072022404601No Hit
GTACTAATACAGTTTACTCTCGCACAGTAATATACGGCCGTGTCCTCAGA8730.10115441168802729No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18850.071.5569151
TCAACGC20500.064.670544
ATCAACG20700.063.6976243
CAACGCA21050.062.6385235
TATCAAC22000.060.261182
AACGCAG22000.059.871176
CGCAGAG26600.049.5175138
ACGCAGA29100.045.263437
GCAGAGT29500.044.649699
GAATACG2600.038.756469
GTCTATA1854.3946784E-935.0907061
TATACGG13800.033.415155
ATACGGC13850.032.740116
CAGCGCT5100.032.459899
TACATGG23600.032.3620762
GGTATCA18400.032.145291
GTACATG24500.031.796481
TACGGCC14900.030.9159857
GTAATAT16250.029.7401261
AATATAC16750.029.2507253
Produced by FastQC (version 0.11.5)